Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 7963..8606 Replicon plasmid p3
Accession NZ_CP006801
Organism Klebsiella pneumoniae subsp. pneumoniae PittNDM01

Toxin (Protein)


Gene name vagD Uniprot ID D8L2J9
Locus tag Q770_RS28180 Protein ID WP_001044770.1
Coordinates 7963..8379 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID D5KTK7
Locus tag Q770_RS28185 Protein ID WP_001261282.1
Coordinates 8376..8606 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Q770_RS28150 3131..3919 + 789 WP_001206315.1 ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1 -
Q770_RS28780 3979..4803 + 825 WP_000722315.1 oxacillin-hydrolyzing class D beta-lactamase OXA-9 -
Q770_RS28160 5003..5320 + 318 Protein_6 recombinase family protein -
Q770_RS28165 5503..6363 + 861 WP_000027057.1 class A broad-spectrum beta-lactamase TEM-1 -
Q770_RS28170 6448..7145 - 698 Protein_8 IS1 family transposase -
Q770_RS28175 7179..7889 - 711 WP_004152334.1 ATP-binding protein -
Q770_RS28180 7963..8379 - 417 WP_001044770.1 type II toxin-antitoxin system VapC family toxin Toxin
Q770_RS28185 8376..8606 - 231 WP_001261282.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
Q770_RS28190 8563..9024 + 462 WP_072202616.1 hypothetical protein -
Q770_RS28195 9168..9518 + 351 WP_000493378.1 hypothetical protein -
Q770_RS28200 9569..10312 + 744 WP_000129823.1 hypothetical protein -
Q770_RS28205 10309..11085 + 777 WP_000015958.1 site-specific integrase -
Q770_RS28210 11143..11400 - 258 WP_000764642.1 hypothetical protein -
Q770_RS32100 11529..11633 - 105 WP_032409716.1 hypothetical protein -
Q770_RS28220 12168..13034 + 867 WP_004118283.1 replication initiation protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / sul1 / qacE / cmlA1 / ere(A) / ARR-3 / blaSHV-187 / aac(3)-IId / blaCTX-M-15 - 1..70814 70814


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15106.52 Da        Isoelectric Point: 7.1084

>T45623 WP_001044770.1 NZ_CP006801:c8379-7963 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Download         Length: 417 bp

>T45623 NZ_CP006801:c8379-7963 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8756.83 Da        Isoelectric Point: 4.4692

>AT45623 WP_001261282.1 NZ_CP006801:c8606-8376 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Download         Length: 231 bp

>AT45623 NZ_CP006801:c8606-8376 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYM2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GZG3

References