Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 7963..8606 | Replicon | plasmid p3 |
| Accession | NZ_CP006801 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae PittNDM01 | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | D8L2J9 |
| Locus tag | Q770_RS28180 | Protein ID | WP_001044770.1 |
| Coordinates | 7963..8379 (-) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | D5KTK7 |
| Locus tag | Q770_RS28185 | Protein ID | WP_001261282.1 |
| Coordinates | 8376..8606 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Q770_RS28150 | 3131..3919 | + | 789 | WP_001206315.1 | ANT(3'')-Ia family aminoglycoside nucleotidyltransferase AadA1 | - |
| Q770_RS28780 | 3979..4803 | + | 825 | WP_000722315.1 | oxacillin-hydrolyzing class D beta-lactamase OXA-9 | - |
| Q770_RS28160 | 5003..5320 | + | 318 | Protein_6 | recombinase family protein | - |
| Q770_RS28165 | 5503..6363 | + | 861 | WP_000027057.1 | class A broad-spectrum beta-lactamase TEM-1 | - |
| Q770_RS28170 | 6448..7145 | - | 698 | Protein_8 | IS1 family transposase | - |
| Q770_RS28175 | 7179..7889 | - | 711 | WP_004152334.1 | ATP-binding protein | - |
| Q770_RS28180 | 7963..8379 | - | 417 | WP_001044770.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| Q770_RS28185 | 8376..8606 | - | 231 | WP_001261282.1 | AbrB/MazE/SpoVT family DNA-binding domain-containing protein | Antitoxin |
| Q770_RS28190 | 8563..9024 | + | 462 | WP_072202616.1 | hypothetical protein | - |
| Q770_RS28195 | 9168..9518 | + | 351 | WP_000493378.1 | hypothetical protein | - |
| Q770_RS28200 | 9569..10312 | + | 744 | WP_000129823.1 | hypothetical protein | - |
| Q770_RS28205 | 10309..11085 | + | 777 | WP_000015958.1 | site-specific integrase | - |
| Q770_RS28210 | 11143..11400 | - | 258 | WP_000764642.1 | hypothetical protein | - |
| Q770_RS32100 | 11529..11633 | - | 105 | WP_032409716.1 | hypothetical protein | - |
| Q770_RS28220 | 12168..13034 | + | 867 | WP_004118283.1 | replication initiation protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Mobilizable plasmid | aac(6')-Ib / ant(3'')-Ia / blaOXA-9 / blaTEM-1A / sul2 / aph(3'')-Ib / aph(6)-Id / sul1 / qacE / cmlA1 / ere(A) / ARR-3 / blaSHV-187 / aac(3)-IId / blaCTX-M-15 | - | 1..70814 | 70814 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15106.52 Da Isoelectric Point: 7.1084
>T45623 WP_001044770.1 NZ_CP006801:c8379-7963 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
Download Length: 417 bp
>T45623 NZ_CP006801:c8379-7963 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8756.83 Da Isoelectric Point: 4.4692
>AT45623 WP_001261282.1 NZ_CP006801:c8606-8376 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT45623 NZ_CP006801:c8606-8376 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GYM2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GZG3 |