Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 3505168..3505793 Replicon chromosome
Accession NZ_CP006798
Organism Klebsiella pneumoniae subsp. pneumoniae PittNDM01

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag Q770_RS17105 Protein ID WP_002882817.1
Coordinates 3505168..3505551 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag Q770_RS17110 Protein ID WP_004150355.1
Coordinates 3505551..3505793 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Q770_RS17090 3502534..3503436 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
Q770_RS17095 3503433..3504068 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
Q770_RS17100 3504065..3504994 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
Q770_RS17105 3505168..3505551 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
Q770_RS17110 3505551..3505793 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
Q770_RS17115 3505998..3506915 + 918 WP_004181612.1 alpha/beta hydrolase -
Q770_RS17120 3506929..3507870 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
Q770_RS17125 3507915..3508352 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
Q770_RS17130 3508349..3509209 - 861 WP_004146232.1 virulence factor BrkB family protein -
Q770_RS17135 3509203..3509802 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T45617 WP_002882817.1 NZ_CP006798:c3505551-3505168 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T45617 NZ_CP006798:c3505551-3505168 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT45617 WP_004150355.1 NZ_CP006798:c3505793-3505551 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT45617 NZ_CP006798:c3505793-3505551 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References