Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 2331570..2332189 Replicon chromosome
Accession NZ_CP006798
Organism Klebsiella pneumoniae subsp. pneumoniae PittNDM01

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag Q770_RS11405 Protein ID WP_002892050.1
Coordinates 2331971..2332189 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag Q770_RS11400 Protein ID WP_002892066.1
Coordinates 2331570..2331944 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Q770_RS11390 2326722..2327915 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
Q770_RS11395 2327938..2331084 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
Q770_RS11400 2331570..2331944 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
Q770_RS11405 2331971..2332189 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
Q770_RS11410 2332348..2332914 + 567 WP_002892030.1 maltose O-acetyltransferase -
Q770_RS31450 2332886..2333026 - 141 WP_004147370.1 hypothetical protein -
Q770_RS11420 2333047..2333517 + 471 WP_002892026.1 YlaC family protein -
Q770_RS11425 2333492..2334943 - 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
Q770_RS11430 2335044..2335742 + 699 WP_004178762.1 GNAT family N-acetyltransferase -
Q770_RS11435 2335739..2335879 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
Q770_RS11440 2335879..2336142 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T45613 WP_002892050.1 NZ_CP006798:2331971-2332189 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T45613 NZ_CP006798:2331971-2332189 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT45613 WP_002892066.1 NZ_CP006798:2331570-2331944 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT45613 NZ_CP006798:2331570-2331944 [Klebsiella pneumoniae subsp. pneumoniae PittNDM01]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References