Detailed information of TA system TA045531

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 35392..35926
• Replicon: plasmid pCD1
• Accession: NZ_CP006753
• Organism: Yersinia pestis 3067

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q663N8
• Locus tag: M482_RS00995
• Protein ID: WP_002213360.1
• Coordinates: 35392..35691 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q9ZGW3
• Locus tag: M482_RS01000
• Protein ID: WP_002224342.1
• Coordinates: 35684..35926 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M482_RS00965	30825..31250	-	426	WP_002213267.1	type III secretion system chaperone SycH	-
M482_RS00970	31627..32025	-	399	Protein_42	ATP-binding protein	-
M482_RS00975	32025..33197	-	1173	WP_002220893.1	IS21 family transposase	-
M482_RS00980	33300..33944	+	645	Protein_44	IS3 family transposase	-
M482_RS00985	34007..34399	-	393	WP_002213403.1	type III secretion system chaperone SycE/YerA	-
M482_RS00990	34593..35252	+	660	WP_002229754.1	type III secretion system effector GTPase activator YopE	-
M482_RS00995	35392..35691	-	300	WP_002213360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M482_RS01000	35684..35926	-	243	WP_002224342.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
M482_RS23450	36092..36384	-	293	Protein_49	IS6 family transposase	-
M482_RS24490	36472..36645	+	174	WP_002213357.1	hypothetical protein	-
M482_RS01005	37100..38065	-	966	WP_002213354.1	ParB/RepB/Spo0J family plasmid partition protein	-
M482_RS01010	38062..39228	-	1167	WP_002220902.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	yscD / yscE / yscF / yscG / yopR/yscH/lcrP / lcrO/yscI / ylpB/yscJ / yscK / yscL / lcrQ/yscM / yopH / yopJ/yopP / yopO/ypkA / yadA / yadA / sycH / sycE/yerA / yopE / yopK/yopQ / yopT / sycT / yopM / yopD / yopB / sycD/lcrH / lcrV / lcrG / lcrR / yscV/lcrD / yscY / yscX / sycN / tyeA / lcrE/yopN / yscN / yscO / yscP / yscQ / yscR / yscS / yscT / yscU / virG/yscW / yscB	1..71522	71522

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (5-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11632.19 Da
• Isoelectric Point: 5.5114

>T45531 WP_002213360.1 NZ_CP006753:c35691-35392 [Yersinia pestis 3067]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE

Nucleotide

• Length: 300 bp

>T45531 NZ_CP006753:c35691-35392 [Yersinia pestis 3067]
GTGTATAAACTGTCTGAACTAGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCCAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGATATTCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGTTCAGGATTTTCCAGAGCATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.15 Da
• Isoelectric Point: 6.4781

>AT45531 WP_002224342.1 NZ_CP006753:c35926-35684 [Yersinia pestis 3067]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT45531 NZ_CP006753:c35926-35684 [Yersinia pestis 3067]
ATGGCTCACGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAATGTACCTGTGAGCCATCGTTTAATGGATGAGATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: Q663N8

Antitoxin

• AlphaFold DB: A0A0E1NQY6