Detailed information of TA system TA045517

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/ParE-CC2985
• Location: 10013..10547
• Replicon: plasmid pCD1
• Accession: NZ_CP006750
• Organism: Yersinia pestis 3770

Toxin (Protein)

• Gene name: parE
• Uniprot ID: Q663N8
• Locus tag: M483_RS00840
• Protein ID: WP_002213360.1
• Coordinates: 10013..10312 (-)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: Q9ZGW3
• Locus tag: M483_RS00845
• Protein ID: WP_002224342.1
• Coordinates: 10305..10547 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
M483_RS00810	5446..5871	-	426	WP_002213267.1	type III secretion system chaperone SycH	-
M483_RS00815	6248..6646	-	399	Protein_7	ATP-binding protein	-
M483_RS00820	6646..7818	-	1173	WP_002220893.1	IS21 family transposase	-
M483_RS00825	7921..8565	+	645	Protein_9	IS3 family transposase	-
M483_RS00830	8628..9020	-	393	WP_002213403.1	type III secretion system chaperone SycE/YerA	-
M483_RS00835	9214..9873	+	660	WP_002229754.1	type III secretion system effector GTPase activator YopE	-
M483_RS00840	10013..10312	-	300	WP_002213360.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
M483_RS00845	10305..10547	-	243	WP_002224342.1	type II toxin-antitoxin system ParD family antitoxin	Antitoxin
M483_RS23440	10713..11005	-	293	Protein_14	IS6 family transposase	-
M483_RS24475	11093..11266	+	174	WP_002213357.1	hypothetical protein	-
M483_RS00850	11721..12686	-	966	WP_002213354.1	ParB/RepB/Spo0J family plasmid partition protein	-
M483_RS00855	12683..13849	-	1167	WP_002220902.1	plasmid-partitioning protein SopA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	sycH / sycE/yerA / yopE / yopK/yopQ / yopT / sycT / yopM / yopD / yopB / sycD/lcrH / lcrV / lcrG / lcrR / yscV/lcrD / yscY / yscX / sycN / tyeA / lcrE/yopN / yscN / yscO / yscP / yscQ / yscR / yscS / yscT / yscU / virG/yscW / yscB / yscC / yscD / yscE / yscF / yscG / yopR/yscH/lcrP / lcrO/yscI / ylpB/yscJ / yscK / yscL / lcrQ/yscM / yopH / yopJ/yopP / yopO/ypkA / yadA / yadA	1..71523	71523

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (2-89)

Antitoxin

• Antitoxin ParD (5-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11632.19 Da
• Isoelectric Point: 5.5114

>T45517 WP_002213360.1 NZ_CP006750:c10312-10013 [Yersinia pestis 3770]
VYKLSELADEDIYNIASYTIRHFGVTQAKLYHENLAKVFELLAKNPELGAECNWICSDIRRFQYKKHGIYYITLSNDILI
SRVLHQSIDIDVQDFPEHE

Nucleotide

• Length: 300 bp

>T45517 NZ_CP006750:c10312-10013 [Yersinia pestis 3770]
GTGTATAAACTGTCTGAACTAGCTGATGAGGATATTTATAATATTGCCAGTTATACTATCCGGCATTTCGGTGTGACTCA
GGCTAAGTTATACCATGAGAACTTGGCAAAGGTATTCGAGCTATTAGCTAAAAATCCAGAGTTAGGGGCTGAGTGTAACT
GGATTTGCTCTGATATTCGCCGTTTTCAGTATAAAAAGCACGGTATATACTATATAACGCTTAGCAATGATATTTTGATT
TCTCGAGTGCTGCATCAATCCATAGATATAGATGTTCAGGATTTTCCAGAGCATGAGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8865.15 Da
• Isoelectric Point: 6.4781

>AT45517 WP_002224342.1 NZ_CP006750:c10547-10305 [Yersinia pestis 3770]
MAHVTSVTLGEHLTGFVGEMIQSGRYGNISEVLRDALRLMEAREQRVQHVRDMVLAGTNVPVSHRLMDEIFSAAVKDTSV

Nucleotide

• Length: 243 bp

>AT45517 NZ_CP006750:c10547-10305 [Yersinia pestis 3770]
ATGGCTCACGTAACCAGCGTAACACTTGGAGAGCATTTGACAGGTTTTGTGGGGGAAATGATTCAGTCTGGTCGTTATGG
CAATATATCAGAAGTGCTTCGTGATGCCTTAAGGCTAATGGAAGCACGTGAACAGCGTGTTCAACATGTACGCGATATGG
TGCTTGCAGGAACAAATGTACCTGTGAGCCATCGTTTAATGGATGAGATTTTTTCTGCTGCGGTGAAAGATACTAGTGTA
TAA

Structures

Toxin

• AlphaFold DB: Q663N8

Antitoxin

• AlphaFold DB: A0A0E1NQY6