Detailed information of TA system TA045505

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/COG3657-dnstrm_HI1420
• Location: 1928246..1928838
• Replicon: chromosome
• Accession: NZ_CP006740
• Organism: Xylella fastidiosa MUL0034

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: A0A060HCK8
• Locus tag: P303_RS08985
• Protein ID: WP_004085590.1
• Coordinates: 1928246..1928542 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: P303_RS08990
• Protein ID: WP_004085592.1
• Coordinates: 1928545..1928838 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P303_RS08965	1924374..1925027	-	654	WP_038211241.1	hypothetical protein	-
P303_RS08970	1925274..1926437	-	1164	WP_040123248.1	phage tail protein	-
P303_RS08975	1926441..1927001	-	561	WP_038230308.1	DUF2612 domain-containing protein	-
P303_RS08980	1926998..1928143	-	1146	WP_011097984.1	baseplate J/gp47 family protein	-
P303_RS08985	1928246..1928542	+	297	WP_004085590.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
P303_RS08990	1928545..1928838	+	294	WP_004085592.1	putative addiction module antidote protein	Antitoxin
P303_RS08995	1928894..1929247	-	354	WP_040123277.1	hypothetical protein	-
P303_RS09000	1929244..1929885	-	642	WP_040123250.1	hypothetical protein	-
P303_RS09005	1929882..1930712	-	831	WP_038230303.1	hypothetical protein	-
P303_RS09010	1930709..1931026	-	318	WP_012382585.1	hypothetical protein	-
P303_RS09015	1931026..1931796	-	771	Protein_1791	hypothetical protein	-
P303_RS09020	1931884..1932183	+	300	WP_024749166.1	type II toxin-antitoxin system RelE/ParE family toxin	-
P303_RS09025	1932187..1932495	+	309	WP_004085003.1	putative addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1881168..1989350	108182

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Uncharacterized protein HI1420 (3-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11169.70 Da
• Isoelectric Point: 10.0610

>T45505 WP_004085590.1 NZ_CP006740:1928246-1928542 [Xylella fastidiosa MUL0034]
MIELKQTDTFRKWREKLKDARARSAIASRLDRLAFGHVGDAEPVGKGVSELRINYGPGYRVYFQRRGDTIYLLLCGGDKG
SQARDIKTALHLSEQWSE

Nucleotide

• Length: 297 bp

>T45505 NZ_CP006740:1928246-1928542 [Xylella fastidiosa MUL0034]
ATGATTGAATTGAAGCAGACTGACACCTTCCGCAAGTGGCGGGAGAAACTCAAGGATGCGCGCGCCCGCTCAGCCATCGC
CTCGCGCCTCGACCGCTTGGCGTTCGGCCATGTCGGCGACGCCGAGCCAGTAGGGAAAGGTGTCAGCGAGCTTCGCATCA
ACTACGGCCCCGGTTACCGGGTGTATTTCCAGCGGCGTGGCGACACGATCTACTTGCTGCTTTGCGGCGGTGACAAAGGA
TCACAAGCGCGCGACATCAAGACTGCGCTGCACCTGTCTGAACAATGGAGCGAATGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10246.59 Da
• Isoelectric Point: 5.6232

>AT45505 WP_004085592.1 NZ_CP006740:1928545-1928838 [Xylella fastidiosa MUL0034]
MTEKLTSYDPAEDLTTDQAIADFMAAAFGTNDPAYVAHALGVVARAKGMTQIASQTGLSREQLYRSFSAEGNPTLRTTLA
VMKALGIELSAKPSGVH

Nucleotide

• Length: 294 bp

>AT45505 NZ_CP006740:1928545-1928838 [Xylella fastidiosa MUL0034]
ATGACCGAGAAACTGACGAGCTACGATCCAGCCGAGGACTTGACCACTGACCAGGCCATCGCCGATTTCATGGCAGCTGC
GTTCGGGACGAACGATCCTGCCTACGTTGCCCACGCGCTGGGCGTCGTTGCCCGCGCCAAGGGCATGACGCAGATCGCCA
GCCAGACAGGGCTATCGCGCGAACAGCTCTACCGCTCGTTCAGTGCCGAGGGCAACCCGACGCTGCGCACAACATTGGCC
GTGATGAAGGCGCTCGGGATCGAGCTGTCTGCGAAACCGTCTGGTGTTCACTGA

Structures

Toxin

• AlphaFold DB: A0A060HCK8