Detailed information of TA system TA045503

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-dnstrm_HI1420
• Location: 1782487..1783092
• Replicon: chromosome
• Accession: NZ_CP006740
• Organism: Xylella fastidiosa MUL0034

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: P303_RS08115
• Protein ID: WP_004089252.1
• Coordinates: 1782487..1782783 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: P303_RS08120
• Protein ID: WP_038211623.1
• Coordinates: 1782787..1783092 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P303_RS13750	1779069..1779221	-	153	WP_162531331.1	hypothetical protein	-
P303_RS08100	1779519..1780682	-	1164	WP_040123248.1	phage tail protein	-
P303_RS08105	1780686..1781246	-	561	WP_038230308.1	DUF2612 domain-containing protein	-
P303_RS08110	1781243..1782388	-	1146	WP_011097984.1	baseplate J/gp47 family protein	-
P303_RS08115	1782487..1782783	+	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
P303_RS08120	1782787..1783092	+	306	WP_038211623.1	putative addiction module antidote protein	Antitoxin
P303_RS08125	1783103..1783456	-	354	WP_040123249.1	hypothetical protein	-
P303_RS08130	1783453..1784094	-	642	WP_040123250.1	hypothetical protein	-
P303_RS08135	1784091..1784921	-	831	WP_038230303.1	hypothetical protein	-
P303_RS08140	1784918..1785235	-	318	WP_012382585.1	hypothetical protein	-
P303_RS08145	1785235..1786005	-	771	Protein_1613	hypothetical protein	-
P303_RS08150	1786093..1786392	+	300	WP_024749166.1	type II toxin-antitoxin system RelE/ParE family toxin	-
P303_RS08155	1786396..1786704	+	309	WP_004085003.1	putative addiction module antidote protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1777388..1827214	49826

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (50-93)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T45503 WP_004089252.1 NZ_CP006740:1782487-1782783 [Xylella fastidiosa MUL0034]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T45503 NZ_CP006740:1782487-1782783 [Xylella fastidiosa MUL0034]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCACGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGGGCTGGCATATCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATCCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTATGGAAGGATTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10982.42 Da
• Isoelectric Point: 5.0941

>AT45503 WP_038211623.1 NZ_CP006740:1782787-1783092 [Xylella fastidiosa MUL0034]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSIDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT45503 NZ_CP006740:1782787-1783092 [Xylella fastidiosa MUL0034]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCTGTCGCTCGTGCTCGTAATTTAA
GTCAACTTGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTATCGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Structures

Toxin

• AlphaFold DB: A0A060HBK9