Detailed information of TA system TA045499

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 836660..837242
• Replicon: chromosome
• Accession: NZ_CP006740
• Organism: Xylella fastidiosa MUL0034

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: A0A060HA48
• Locus tag: P303_RS03765
• Protein ID: WP_038229226.1
• Coordinates: 836660..836950 (+)
• Length: 97 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: A0A060HE88
• Locus tag: P303_RS03770
• Protein ID: WP_004091409.1
• Coordinates: 836937..837242 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P303_RS03735	832353..833510	-	1158	WP_038229247.1	transposase	-
P303_RS03740	833533..833949	+	417	WP_038229223.1	IS200/IS605 family transposase	-
P303_RS03745	834376..835281	+	906	WP_196242989.1	hypothetical protein	-
P303_RS03750	835423..835749	-	327	WP_004091396.1	DNA-binding protein	-
P303_RS03755	835879..836199	-	321	WP_004091397.1	type II toxin-antitoxin system RelE/ParE family toxin	-
P303_RS03760	836310..836483	-	174	Protein_720	XRE family transcriptional regulator	-
P303_RS03765	836660..836950	+	291	WP_038229226.1	BrnT family toxin	Toxin
P303_RS03770	836937..837242	+	306	WP_004091409.1	BrnA antitoxin family protein	Antitoxin
P303_RS03775	837579..838484	-	906	WP_040123139.1	AEC family transporter	-
P303_RS12480	838495..839394	-	900	WP_140189706.1	TauD/TfdA family dioxygenase	-
P303_RS12485	839593..839757	-	165	Protein_725	Tn3 family transposase	-
P303_RS03790	839990..840232	+	243	WP_004085758.1	type II toxin-antitoxin system ParD family antitoxin	-
P303_RS03795	840225..840512	+	288	WP_038229230.1	type II toxin-antitoxin system RelE/ParE family toxin	-
P303_RS03800	840600..841166	+	567	WP_038229232.1	recombinase family protein	-
P303_RS03805	841251..841460	-	210	WP_040123140.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	840600..841166	566

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-89)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (11-93)

Sequences

Toxin

Protein

• Length: 97 a.a.
• Molecular weight: 11304.62 Da
• Isoelectric Point: 6.0917

>T45499 WP_038229226.1 NZ_CP006740:836660-836950 [Xylella fastidiosa MUL0034]
VERYFEWDDKKAESNFRKHGIRFDEAALAFDDPFAVSEQNRIENGEQRWQTIGMAGGCLLLLVAHTVHFEDDGIEVVRII
SARRVDRKERRRYEHG

Nucleotide

• Length: 291 bp

>T45499 NZ_CP006740:836660-836950 [Xylella fastidiosa MUL0034]
GTGGAACGATATTTCGAGTGGGACGACAAGAAGGCGGAAAGCAACTTCCGTAAGCACGGCATCCGGTTCGATGAAGCTGC
GCTTGCCTTCGACGACCCGTTTGCAGTGTCGGAACAAAACCGTATCGAAAATGGGGAGCAGCGTTGGCAAACCATCGGAA
TGGCCGGTGGGTGCCTGCTGCTGCTGGTGGCGCACACAGTGCACTTTGAAGATGATGGCATCGAGGTTGTACGAATCATC
AGTGCCCGTCGCGTGGACCGAAAGGAACGGAGGCGTTATGAACATGGTTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11684.43 Da
• Isoelectric Point: 9.4870

>AT45499 WP_004091409.1 NZ_CP006740:836937-837242 [Xylella fastidiosa MUL0034]
MNMVRYKQSELPPLTEERKAELKALAKKPDSEIDYSDIPPLDESFWARAVPNPFFKPVKTHASVRIDSDVLAWLKSQGKG
YQTRMNAILREAMLRSLHQQE

Nucleotide

• Length: 306 bp

>AT45499 NZ_CP006740:836937-837242 [Xylella fastidiosa MUL0034]
ATGAACATGGTTAGGTACAAGCAGAGCGAGTTGCCGCCGCTGACCGAAGAACGTAAGGCAGAACTCAAGGCGCTAGCAAA
GAAGCCAGATAGCGAAATCGACTACAGCGATATTCCACCGTTGGATGAATCGTTTTGGGCGCGGGCAGTACCCAACCCAT
TCTTTAAGCCGGTCAAGACACATGCTTCTGTGCGTATTGATTCGGATGTGTTGGCTTGGCTCAAGAGTCAAGGCAAGGGC
TACCAGACCCGTATGAATGCAATCTTGCGAGAAGCCATGCTTCGCTCGCTGCACCAGCAAGAATAA

Structures

Toxin

• AlphaFold DB: A0A060HA48

Antitoxin

• AlphaFold DB: A0A060HE88