Detailed information of TA system TA045475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 32321..32895
• Replicon: plasmid lcp2
• Accession: NZ_CP006726
• Organism: Leptospira interrogans serovar Linhai str. 56609

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A0C5XJY0
• Locus tag: LIL_RS20630
• Protein ID: WP_000240419.1
• Coordinates: 32551..32895 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: M6U553
• Locus tag: LIL_RS20625
• Protein ID: WP_000443825.1
• Coordinates: 32321..32557 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LIL_RS20605	27960..28898	-	939	WP_045193752.1	Rpn family recombination-promoting nuclease/putative transposase	-
LIL_RS20610	29170..30087	-	918	WP_002109360.1	leucine-rich repeat domain-containing protein	-
LIL_RS23900	30312..30526	-	215	Protein_17	hypothetical protein	-
LIL_RS20615	31302..31514	+	213	WP_000447736.1	hypothetical protein	-
LIL_RS20620	31563..31985	+	423	WP_000402980.1	hypothetical protein	-
LIL_RS20625	32321..32557	+	237	WP_000443825.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LIL_RS20630	32551..32895	+	345	WP_000240419.1	endoribonuclease MazF	Toxin
LIL_RS20650	34560..34883	+	324	WP_002078026.1	hypothetical protein	-
LIL_RS20660	35432..35665	+	234	WP_045193758.1	nucleotidyltransferase domain-containing protein	-
LIL_RS20665	35655..35894	+	240	WP_045193760.1	antitoxin	-
LIL_RS20670	36254..36700	+	447	WP_000445079.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..56757	56757

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (11-111)

Antitoxin

• Antitoxin PemI-like (1-78)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12985.21 Da
• Isoelectric Point: 9.9595

>T45475 WP_000240419.1 NZ_CP006726:32551-32895 [Leptospira interrogans serovar Linhai str. 56609]
MVKNRNYTPEKGDIVWLNFTPQAGHEQKGRRPALVLSPKEYNSKTGLAIFCPITSKIKGYPFEVLIKSKKIDGVILSDQV
KNLDWTIREAEFIESINKVSLKEVLDNIKLLILT

Nucleotide

• Length: 345 bp

>T45475 NZ_CP006726:32551-32895 [Leptospira interrogans serovar Linhai str. 56609]
TTGGTAAAGAATAGAAACTATACTCCTGAAAAAGGAGATATAGTGTGGCTGAACTTTACTCCTCAAGCAGGTCATGAACA
GAAAGGTCGTAGACCTGCACTTGTTTTATCGCCTAAGGAATATAATTCAAAGACAGGGTTAGCAATATTTTGTCCAATTA
CCAGTAAAATTAAAGGTTATCCATTCGAAGTGTTGATTAAATCAAAAAAAATCGATGGTGTAATTCTTTCTGATCAAGTA
AAAAACTTAGATTGGACTATCCGTGAAGCAGAATTTATTGAATCAATAAATAAAGTTTCTTTAAAAGAAGTTTTAGATAA
TATTAAATTATTAATATTAACCTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8728.95 Da
• Isoelectric Point: 5.6663

>AT45475 WP_000443825.1 NZ_CP006726:32321-32557 [Leptospira interrogans serovar Linhai str. 56609]
MESIIQKWGNSLGIRIPKAMATELELNDGSHVELQYEGDKIVIYPMKKASLEDKLSKITKQNLHSEISTGNSIGNEAW

Nucleotide

• Length: 237 bp

>AT45475 NZ_CP006726:32321-32557 [Leptospira interrogans serovar Linhai str. 56609]
ATGGAATCAATTATTCAAAAATGGGGTAATAGCTTAGGAATTAGAATTCCAAAAGCAATGGCAACGGAATTAGAGTTAAA
TGATGGAAGCCATGTTGAATTACAATATGAAGGAGATAAGATTGTAATATATCCAATGAAAAAAGCTTCATTGGAAGATA
AACTTTCTAAAATTACGAAACAAAATCTTCACTCTGAAATATCTACTGGTAATTCTATAGGCAATGAAGCTTGGTAA

Structures

Toxin

• AlphaFold DB: A0A0C5XJY0

Antitoxin

• AlphaFold DB: A0A0F6HC33