Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazE-MazF
Location 40480..41045 Replicon plasmid lcp1
Accession NZ_CP006725
Organism Leptospira interrogans serovar Linhai str. 56609

Toxin (Protein)


Gene name mazF Uniprot ID A0A0F6H5X2
Locus tag LIL_RS20395 Protein ID WP_000648189.1
Coordinates 40480..40728 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A0C5XAD8
Locus tag LIL_RS20400 Protein ID WP_000237066.1
Coordinates 40722..41045 (+) Length 108 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIL_RS20350 35540..35902 + 363 WP_000821938.1 hypothetical protein -
LIL_RS20355 35895..36275 + 381 WP_045193734.1 hypothetical protein -
LIL_RS20360 36275..36718 + 444 WP_045193722.1 hypothetical protein -
LIL_RS20365 36725..37186 + 462 WP_001115249.1 hypothetical protein -
LIL_RS20370 37186..37620 + 435 WP_002110375.1 M15 family metallopeptidase -
LIL_RS20375 37825..38058 + 234 WP_045193725.1 hypothetical protein -
LIL_RS20380 38360..38548 + 189 WP_000425065.1 hypothetical protein -
LIL_RS20385 39211..39516 + 306 WP_000844596.1 hypothetical protein -
LIL_RS23860 39489..40175 + 687 WP_082271315.1 restriction endonuclease -
LIL_RS20395 40480..40728 + 249 WP_000648189.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Toxin
LIL_RS20400 40722..41045 + 324 WP_000237066.1 type II toxin-antitoxin system PemK/MazF family toxin Antitoxin
LIL_RS20405 41168..41338 + 171 WP_002077237.1 hypothetical protein -
LIL_RS20410 41422..41799 - 378 WP_000603456.1 hypothetical protein -
LIL_RS24735 42413..42553 + 141 WP_002077221.1 hypothetical protein -
LIL_RS20415 42729..43182 + 454 Protein_53 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..67282 67282


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

SpoVT-AbrB domain

(6-49)

Antitoxin

mRNA interferase PemK-like

(2-105)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9507.04 Da        Isoelectric Point: 5.1749

>T45473 WP_000648189.1 NZ_CP006725:40480-40728 [Leptospira interrogans serovar Linhai str. 56609]
MKASVVKIGNSKGIRIPKAVLEECHIEEEVDLLIDNNKLIIVPLKSKPREGWEKQFKTMSSNKDDKLFIPDSIDLLDKDW
EW

Download         Length: 249 bp

>T45473 NZ_CP006725:40480-40728 [Leptospira interrogans serovar Linhai str. 56609]
ATGAAGGCTTCAGTCGTAAAAATAGGCAATTCCAAAGGTATTCGAATTCCCAAGGCAGTCTTAGAAGAATGTCATATTGA
AGAAGAAGTAGATTTACTAATCGATAATAACAAATTAATTATTGTACCTCTAAAATCGAAACCAAGAGAAGGGTGGGAAA
AACAATTTAAAACAATGTCATCTAATAAAGATGATAAATTATTTATTCCTGATTCAATAGATTTATTAGACAAGGATTGG
GAATGGTGA

Antitoxin


Download         Length: 108 a.a.        Molecular weight: 12168.59 Da        Isoelectric Point: 10.5161

>AT45473 WP_000237066.1 NZ_CP006725:40722-41045 [Leptospira interrogans serovar Linhai str. 56609]
MVISQYEVYLINLDPTVGHEIKKSRPCAIISPNEMNKTIGTIIIAPMTTKSRSYPTRVELTFQGKKGWIVLDQIRAVDKT
RLVKKLGKIDPKTVNKMKLVIKEMLVD

Download         Length: 324 bp

>AT45473 NZ_CP006725:40722-41045 [Leptospira interrogans serovar Linhai str. 56609]
ATGGTGATTTCTCAATACGAAGTATATCTAATAAATTTAGATCCAACGGTTGGACATGAGATAAAAAAATCAAGACCTTG
TGCAATAATCTCACCCAATGAAATGAATAAAACGATTGGAACAATTATTATTGCACCAATGACTACAAAATCTCGATCAT
ATCCGACAAGAGTTGAATTAACCTTTCAAGGAAAGAAGGGTTGGATTGTGTTAGATCAGATTCGAGCTGTCGATAAAACA
CGTCTGGTAAAGAAGTTAGGAAAAATAGATCCCAAAACAGTTAATAAAATGAAACTGGTAATAAAGGAAATGTTGGTTGA
TTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6H5X2

Antitoxin

Source ID Structure
AlphaFold DB A0A0C5XAD8

References