45472

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1-VagC
Location 3274274..3274902 Replicon chromosome
Accession NZ_CP006723
Organism Leptospira interrogans serovar Linhai str. 56609

Toxin (Protein)


Gene name vapC Uniprot ID Q8F7E4
Locus tag LIL_RS14065 Protein ID WP_000281042.1
Coordinates 3274504..3274902 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID Q8F7E3
Locus tag LIL_RS14060 Protein ID WP_001192482.1
Coordinates 3274274..3274504 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIL_RS21445 3269581..3269922 + 342 Protein_2631 tyrosine-type recombinase/integrase -
LIL_RS14040 3269977..3271092 + 1116 WP_000353261.1 protein kinase -
LIL_RS14045 3271297..3272220 + 924 WP_000680715.1 M28 family peptidase -
LIL_RS14050 3272428..3273117 + 690 WP_000590463.1 SH3 domain-containing protein -
LIL_RS14055 3273349..3274026 + 678 WP_000071998.1 hypothetical protein -
LIL_RS14060 3274274..3274504 + 231 WP_001192482.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LIL_RS14065 3274504..3274902 + 399 WP_000281042.1 type II toxin-antitoxin system VapC family toxin Toxin
LIL_RS14070 3275000..3275548 + 549 WP_000479568.1 hypothetical protein -
LIL_RS14075 3275691..3276767 + 1077 WP_045192729.1 hypothetical protein -
LIL_RS14080 3276801..3277226 + 426 WP_000558071.1 GNAT family N-acetyltransferase -
LIL_RS14085 3277443..3278303 - 861 WP_000682231.1 hypothetical protein -
LIL_RS14090 3278815..3279582 - 768 WP_000636952.1 DUF1554 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15139.76 Da        Isoelectric Point: 9.6893

>T45472 WP_000281042.1 NZ_CP006723:3274504-3274902 [Leptospira interrogans serovar Linhai str. 56609]
MYLLDTNICIFLIKKKNATLLENLKKKLNKDLFVSSLTVAELEFGIQKSEFKEKNKVALIEFLTIFNILSFSDKDAESYG
IIRADLERKGNVIGSIDMLLAAQAIANNYIFVTNNTKEFKRIKALKIENWTQ

Download         Length: 399 bp

>T45472 NZ_CP006723:3274504-3274902 [Leptospira interrogans serovar Linhai str. 56609]
ATGTATCTTTTAGATACCAATATTTGTATTTTTCTAATTAAAAAGAAAAATGCGACTCTGTTAGAAAATCTTAAGAAAAA
ATTAAATAAAGATCTATTTGTTTCGTCTTTAACTGTTGCGGAACTTGAGTTTGGAATTCAGAAAAGTGAATTTAAAGAAA
AGAATAAAGTTGCACTAATCGAATTTTTAACGATTTTCAATATACTTTCCTTTTCTGATAAAGATGCAGAGTCTTACGGT
ATCATCAGAGCGGATTTGGAGAGGAAAGGTAATGTAATCGGTTCAATAGACATGTTACTTGCCGCTCAAGCAATTGCTAA
TAATTATATCTTTGTTACTAATAATACAAAAGAATTTAAAAGAATTAAGGCTTTAAAAATAGAAAATTGGACACAGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8771.05 Da        Isoelectric Point: 8.6588

>AT45472 WP_001192482.1 NZ_CP006723:3274274-3274504 [Leptospira interrogans serovar Linhai str. 56609]
MQTAKLFINGRSQAVRLPKEFQFTGDDVLIQKVGEAVILVPKNKAWNVFLEGLNGFSDDFFKEEREQPKSDKREKL

Download         Length: 231 bp

>AT45472 NZ_CP006723:3274274-3274504 [Leptospira interrogans serovar Linhai str. 56609]
ATGCAAACAGCCAAATTATTTATAAATGGAAGAAGTCAAGCGGTCAGATTACCAAAAGAATTTCAATTTACCGGAGATGA
TGTTCTCATTCAAAAAGTCGGAGAGGCGGTAATATTGGTTCCGAAAAACAAGGCCTGGAACGTATTTTTAGAAGGATTGA
ACGGGTTTTCAGATGATTTCTTTAAGGAGGAGAGAGAACAGCCGAAATCTGATAAAAGAGAAAAACTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0F6HAN6

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HAF7

References