Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) chpIK/PemK(toxin)
Location 1543776..1544352 Replicon chromosome
Accession NZ_CP006723
Organism Leptospira interrogans serovar Linhai str. 56609

Toxin (Protein)


Gene name chpK Uniprot ID Q93MT8
Locus tag LIL_RS06700 Protein ID WP_000617906.1
Coordinates 1544011..1544352 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name chpI Uniprot ID Q93MT9
Locus tag LIL_RS06695 Protein ID WP_000844758.1
Coordinates 1543776..1544024 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIL_RS06680 1539431..1541413 + 1983 WP_045191802.1 HD family phosphohydrolase -
LIL_RS24540 1542055..1542509 - 455 Protein_1249 hypothetical protein -
LIL_RS06690 1542663..1542959 + 297 WP_000477061.1 helix-turn-helix domain-containing protein -
LIL_RS06695 1543776..1544024 + 249 WP_000844758.1 ribbon-helix-helix domain-containing protein Antitoxin
LIL_RS06700 1544011..1544352 + 342 WP_000617906.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
LIL_RS06705 1545375..1546367 - 993 WP_000041092.1 aldolase -
LIL_RS06710 1546507..1547442 - 936 WP_000004531.1 ornithine carbamoyltransferase -
LIL_RS06720 1548246..1548713 + 468 WP_000060052.1 hypothetical protein -
LIL_RS06725 1548710..1549114 + 405 WP_001974506.1 pyridoxamine 5'-phosphate oxidase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-108)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(2-41)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 12372.25 Da        Isoelectric Point: 6.3272

>T45471 WP_000617906.1 NZ_CP006723:1544011-1544352 [Leptospira interrogans serovar Linhai str. 56609]
MIRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNQSNINTIVSIAITSNLNLSEAPGNVFISKKDSSLSKDSVINVSQIV
TLDKERFLNKAGKLKSNKLGEVEIGLKLVTGLD

Download         Length: 342 bp

>T45471 NZ_CP006723:1544011-1544352 [Leptospira interrogans serovar Linhai str. 56609]
ATGATTCGTGGTGAAATTTGGTGGGTAGATTTGGGAATTCCATTTGGAAGTGAGCCTGGATTTAAACGTCCAGTTCTTAT
AATTCAAGATGACTCTTTTAATCAAAGTAATATTAATACAATAGTTTCGATTGCAATTACATCGAATTTAAATCTATCTG
AAGCACCAGGTAATGTTTTCATTAGTAAAAAAGATTCGAGTTTATCAAAGGACTCGGTCATAAATGTCTCTCAAATTGTG
ACTTTAGATAAAGAAAGATTTCTTAACAAAGCGGGTAAACTTAAATCAAATAAATTAGGTGAAGTTGAAATTGGGCTTAA
GTTGGTTACAGGCTTAGATTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9380.77 Da        Isoelectric Point: 10.3815

>AT45471 WP_000844758.1 NZ_CP006723:1543776-1544024 [Leptospira interrogans serovar Linhai str. 56609]
MKTAISIPDELFRAAEKIAKKLGIPRSQLFAKALEEFIQSHSKESVTEKLNKIYSNKSKETRNNITDLSVESLRKSLKND
SW

Download         Length: 249 bp

>AT45471 NZ_CP006723:1543776-1544024 [Leptospira interrogans serovar Linhai str. 56609]
ATGAAGACGGCGATTTCAATTCCTGATGAGTTATTTAGGGCTGCCGAGAAAATAGCTAAAAAACTTGGGATTCCTCGAAG
CCAGTTATTTGCAAAAGCATTAGAAGAATTTATCCAATCTCATAGTAAAGAATCTGTAACTGAGAAATTAAATAAAATCT
ACAGTAATAAATCCAAAGAAACTAGAAATAATATTACTGACTTATCCGTAGAATCGCTTCGTAAGAGTTTAAAAAATGAT
TCGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M4MS74

Antitoxin

Source ID Structure
AlphaFold DB A0A0F6HD92

References