Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5262875..5263500 Replicon chromosome
Accession NZ_CP006722
Organism Klebsiella pneumoniae subsp. pneumoniae 1158

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag P243_RS25575 Protein ID WP_002882817.1
Coordinates 5262875..5263258 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag P243_RS25580 Protein ID WP_004150355.1
Coordinates 5263258..5263500 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P243_RS25560 5260241..5261143 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
P243_RS25565 5261140..5261775 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
P243_RS25570 5261772..5262701 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
P243_RS25575 5262875..5263258 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
P243_RS25580 5263258..5263500 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
P243_RS25585 5263705..5264622 + 918 WP_029884108.1 alpha/beta hydrolase -
P243_RS25590 5264636..5265577 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
P243_RS25595 5265622..5266059 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
P243_RS25600 5266056..5266916 - 861 WP_002882807.1 virulence factor BrkB family protein -
P243_RS25605 5266910..5267509 - 600 WP_004901669.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T45470 WP_002882817.1 NZ_CP006722:c5263258-5262875 [Klebsiella pneumoniae subsp. pneumoniae 1158]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T45470 NZ_CP006722:c5263258-5262875 [Klebsiella pneumoniae subsp. pneumoniae 1158]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCAGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT45470 WP_004150355.1 NZ_CP006722:c5263500-5263258 [Klebsiella pneumoniae subsp. pneumoniae 1158]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT45470 NZ_CP006722:c5263500-5263258 [Klebsiella pneumoniae subsp. pneumoniae 1158]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References