Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4046315..4046934 Replicon chromosome
Accession NZ_CP006722
Organism Klebsiella pneumoniae subsp. pneumoniae 1158

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag P243_RS19620 Protein ID WP_002892050.1
Coordinates 4046716..4046934 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag P243_RS19615 Protein ID WP_002892066.1
Coordinates 4046315..4046689 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
P243_RS19605 4041467..4042660 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
P243_RS19610 4042683..4045829 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
P243_RS19615 4046315..4046689 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
P243_RS19620 4046716..4046934 + 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
P243_RS19625 4047093..4047659 + 567 WP_002892030.1 maltose O-acetyltransferase -
P243_RS28795 4047631..4047771 - 141 WP_004147370.1 hypothetical protein -
P243_RS19635 4047792..4048262 + 471 WP_002892026.1 YlaC family protein -
P243_RS19640 4048269..4049726 - 1458 WP_029883993.1 PLP-dependent aminotransferase family protein -
P243_RS19645 4049827..4050525 + 699 WP_002892021.1 GNAT family N-acetyltransferase -
P243_RS19650 4050522..4050662 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
P243_RS19655 4050662..4050925 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T45467 WP_002892050.1 NZ_CP006722:4046716-4046934 [Klebsiella pneumoniae subsp. pneumoniae 1158]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T45467 NZ_CP006722:4046716-4046934 [Klebsiella pneumoniae subsp. pneumoniae 1158]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT45467 WP_002892066.1 NZ_CP006722:4046315-4046689 [Klebsiella pneumoniae subsp. pneumoniae 1158]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT45467 NZ_CP006722:4046315-4046689 [Klebsiella pneumoniae subsp. pneumoniae 1158]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References