Detailed information of TA system TA045454

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 201265..202004
• Replicon: chromosome
• Accession: NZ_CP006722
• Organism: Klebsiella pneumoniae subsp. pneumoniae 1158

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: P243_RS00990
• Protein ID: WP_039109138.1
• Coordinates: 201519..202004 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A7D3T1D0
• Locus tag: P243_RS00985
• Protein ID: WP_004213599.1
• Coordinates: 201265..201531 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
P243_RS00970	196769..198838	+	2070	WP_004901297.1	glycine--tRNA ligase subunit beta	-
P243_RS27340	199134..200503	+	1370	WP_087661561.1	IS3 family transposase	-
P243_RS26160	200704..201132	+	429	WP_004901287.1	GFA family protein	-
P243_RS00985	201265..201531	+	267	WP_004213599.1	DUF1778 domain-containing protein	Antitoxin
P243_RS00990	201519..202004	+	486	WP_039109138.1	GNAT family N-acetyltransferase	Toxin
P243_RS00995	202329..203375	-	1047	WP_022615678.1	IS481-like element ISKpn28 family transposase	-
P243_RS01000	203449..203601	+	153	WP_002922102.1	type I toxin-antitoxin system toxin HokA	-
P243_RS01005	203903..205522	+	1620	WP_029884695.1	ATP-binding cassette domain-containing protein	-
P243_RS01010	205621..205833	-	213	WP_000014594.1	RNA chaperone/antiterminator CspA	-
P243_RS01015	206085..206375	-	291	WP_002921931.1	HTH-type transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	-	-	199871..203375	3504

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (66-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17450.19 Da
• Isoelectric Point: 10.2935

>T45454 WP_039109138.1 NZ_CP006722:201519-202004 [Klebsiella pneumoniae subsp. pneumoniae 1158]
VGRITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKKGTKKVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTQPRTLFLRLP
Q

Nucleotide

• Length: 486 bp

>T45454 NZ_CP006722:201519-202004 [Klebsiella pneumoniae subsp. pneumoniae 1158]
GTGGGACGTATAACCGCGCCCGAGCCGTTATCCAGCGCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCACTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGAAGGGTACGA
AGAAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCTACAGGCAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGTGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGCGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCAGCCGCGGACACTGTTCCTCAGACTCCCT
CAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10151.58 Da
• Isoelectric Point: 4.5141

>AT45454 WP_004213599.1 NZ_CP006722:201265-201531 [Klebsiella pneumoniae subsp. pneumoniae 1158]
MKSDVQLNLRAKESQRVLIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDDSAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT45454 NZ_CP006722:201265-201531 [Klebsiella pneumoniae subsp. pneumoniae 1158]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGTACTCATCGATGCCGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATCCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGATTCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7D3T1D0