Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/DinJ(antitoxin)
Location 1090619..1091255 Replicon chromosome
Accession NZ_CP006716
Organism Bifidobacterium breve S27

Toxin (Protein)


Gene name mazF Uniprot ID A0A8U0K1H5
Locus tag BS27_RS04500 Protein ID WP_025341719.1
Coordinates 1090619..1090975 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag BS27_RS04505 Protein ID WP_025341720.1
Coordinates 1090962..1091255 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BS27_RS04470 1085806..1086717 + 912 WP_025341717.1 ABC transporter ATP-binding protein -
BS27_RS04475 1086914..1087540 + 627 WP_003829226.1 30S ribosomal protein S4 -
BS27_RS04480 1087877..1088659 - 783 WP_003829227.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
BS27_RS04485 1088801..1089286 - 486 WP_025341718.1 cytidine deaminase -
BS27_RS04490 1089379..1089735 + 357 WP_012577924.1 SdpI family protein -
BS27_RS04495 1089840..1090442 + 603 WP_003829233.1 transglutaminase family protein -
BS27_RS04500 1090619..1090975 - 357 WP_025341719.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
BS27_RS04505 1090962..1091255 - 294 WP_025341720.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
BS27_RS04510 1091406..1091570 - 165 Protein_884 IS30 family transposase -
BS27_RS04515 1091670..1092344 + 675 WP_015438748.1 histidine phosphatase family protein -
BS27_RS04520 1092427..1092831 + 405 WP_003829238.1 DUF948 domain-containing protein -
BS27_RS04525 1092831..1093067 + 237 WP_003829239.1 hypothetical protein -
BS27_RS04530 1093163..1095841 + 2679 WP_003829240.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13286.20 Da        Isoelectric Point: 7.9984

>T45453 WP_025341719.1 NZ_CP006716:c1090975-1090619 [Bifidobacterium breve S27]
MMKTDPHQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T45453 NZ_CP006716:c1090975-1090619 [Bifidobacterium breve S27]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10896.39 Da        Isoelectric Point: 4.2896

>AT45453 WP_025341720.1 NZ_CP006716:c1091255-1090962 [Bifidobacterium breve S27]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFMPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT45453 NZ_CP006716:c1091255-1090962 [Bifidobacterium breve S27]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCATGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References