Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1096327..1096963 Replicon chromosome
Accession NZ_CP006715
Organism Bifidobacterium breve 689b

Toxin (Protein)


Gene name mazF Uniprot ID W6EZW1
Locus tag B689B_RS04585 Protein ID WP_014484977.1
Coordinates 1096327..1096683 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag B689B_RS04590 Protein ID WP_012577921.1
Coordinates 1096670..1096963 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B689B_RS04555 1091519..1092427 + 909 WP_025332235.1 ABC transporter ATP-binding protein -
B689B_RS04560 1092624..1093250 + 627 WP_003829226.1 30S ribosomal protein S4 -
B689B_RS04565 1093587..1094369 - 783 WP_015438747.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
B689B_RS04570 1094512..1094997 - 486 WP_025332236.1 cytidine deaminase -
B689B_RS04575 1095087..1095443 + 357 WP_025332237.1 SdpI family protein -
B689B_RS04580 1095548..1096150 + 603 WP_003829233.1 transglutaminase family protein -
B689B_RS04585 1096327..1096683 - 357 WP_014484977.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
B689B_RS04590 1096670..1096963 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
B689B_RS04595 1097397..1098071 + 675 WP_015438748.1 histidine phosphatase family protein -
B689B_RS04600 1098154..1098558 + 405 WP_003829238.1 DUF948 domain-containing protein -
B689B_RS04605 1098558..1098794 + 237 WP_003829239.1 hypothetical protein -
B689B_RS04610 1098890..1101568 + 2679 WP_003829240.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-111)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13302.25 Da        Isoelectric Point: 8.9603

>T45450 WP_014484977.1 NZ_CP006715:c1096683-1096327 [Bifidobacterium breve 689b]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVAWAINELYPGLLA

Download         Length: 357 bp

>T45450 NZ_CP006715:c1096683-1096327 [Bifidobacterium breve 689b]
ATGATGAAGACTGATCCGCGCCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAATCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCGCCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT45450 WP_012577921.1 NZ_CP006715:c1096963-1096670 [Bifidobacterium breve 689b]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT45450 NZ_CP006715:c1096963-1096670 [Bifidobacterium breve 689b]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7D489

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References