Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 984928..985483 Replicon chromosome
Accession NZ_CP006714
Organism Bifidobacterium breve NCFB 2258

Toxin (Protein)


Gene name mazF Uniprot ID B7GSS5
Locus tag B2258_RS04045 Protein ID WP_003833460.1
Coordinates 984928..985251 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag B2258_RS04050 Protein ID WP_003829112.1
Coordinates 985238..985483 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B2258_RS10125 979965..980253 - 289 Protein_787 IS256 family transposase -
B2258_RS04025 980724..981989 - 1266 WP_025262925.1 Fic family protein -
B2258_RS04030 982531..982896 - 366 WP_013140585.1 YccF domain-containing protein -
B2258_RS04035 983134..984003 + 870 WP_003829104.1 class I SAM-dependent methyltransferase -
B2258_RS10135 984097..984711 + 615 WP_003829107.1 cupin domain-containing protein -
B2258_RS04045 984928..985251 - 324 WP_003833460.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
B2258_RS04050 985238..985483 - 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
B2258_RS04055 985657..986913 + 1257 WP_003829116.1 HipA domain-containing protein -
B2258_RS04060 987178..987369 + 192 WP_003829118.1 hypothetical protein -
B2258_RS04065 987553..988824 - 1272 WP_003829121.1 MFS transporter -
B2258_RS04070 988981..989643 - 663 WP_025262926.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 974698..1022983 48285


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-106)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11829.64 Da        Isoelectric Point: 6.2932

>T45447 WP_003833460.1 NZ_CP006714:c985251-984928 [Bifidobacterium breve NCFB 2258]
MKRGEIRTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T45447 NZ_CP006714:c985251-984928 [Bifidobacterium breve NCFB 2258]
ATGAAACGAGGTGAGATTCGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT45447 WP_003829112.1 NZ_CP006714:c985483-985238 [Bifidobacterium breve NCFB 2258]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT45447 NZ_CP006714:c985483-985238 [Bifidobacterium breve NCFB 2258]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4R0SL31

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References