Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/DinJ(antitoxin)
Location 1118271..1118904 Replicon chromosome
Accession NZ_CP006713
Organism Bifidobacterium breve JCM 7019

Toxin (Protein)


Gene name mazF Uniprot ID W6EYF0
Locus tag B7019_RS04780 Protein ID WP_012577854.1
Coordinates 1118536..1118904 (+) Length 123 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID W6F017
Locus tag B7019_RS04775 Protein ID WP_025221597.1
Coordinates 1118271..1118555 (+) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7019_RS04730 1113885..1114112 + 228 WP_025221588.1 helix-turn-helix domain-containing protein -
B7019_RS04735 1114325..1114939 + 615 WP_025221589.1 lysozyme -
B7019_RS11130 1115029..1115190 + 162 WP_155245937.1 hypothetical protein -
B7019_RS04740 1115187..1115531 + 345 WP_025221590.1 hypothetical protein -
B7019_RS04745 1115643..1116023 + 381 WP_025221591.1 hypothetical protein -
B7019_RS11135 1116020..1116190 + 171 WP_155266422.1 hypothetical protein -
B7019_RS04750 1116222..1116980 + 759 WP_025221592.1 hypothetical protein -
B7019_RS04755 1116982..1117323 + 342 WP_025221593.1 hypothetical protein -
B7019_RS04760 1117323..1117505 + 183 WP_025221594.1 hypothetical protein -
B7019_RS04765 1117518..1117811 + 294 WP_025221595.1 hypothetical protein -
B7019_RS04770 1117896..1118198 + 303 WP_025221596.1 hypothetical protein -
B7019_RS04775 1118271..1118555 + 285 WP_025221597.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
B7019_RS04780 1118536..1118904 + 369 WP_012577854.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
B7019_RS04785 1119093..1119638 + 546 WP_025221598.1 hypothetical protein -
B7019_RS04790 1119635..1119862 + 228 WP_014484964.1 hypothetical protein -
B7019_RS04795 1119859..1120629 + 771 WP_025221599.1 hypothetical protein -
B7019_RS04800 1121003..1121218 - 216 WP_025221600.1 hypothetical protein -
B7019_RS04805 1121215..1121715 - 501 WP_025221601.1 hypothetical protein -
B7019_RS04810 1121712..1122224 - 513 WP_025221602.1 hypothetical protein -
B7019_RS04815 1122221..1122499 - 279 WP_025221603.1 hypothetical protein -
B7019_RS04820 1122566..1122817 - 252 WP_025221604.1 hypothetical protein -
B7019_RS04825 1122814..1123008 - 195 WP_025221605.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 1084304..1142665 58361


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(16-111)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-53)


Sequences

Toxin        


Download         Length: 123 a.a.        Molecular weight: 13866.75 Da        Isoelectric Point: 4.5746

>T45445 WP_012577854.1 NZ_CP006713:1118536-1118904 [Bifidobacterium breve JCM 7019]
MTSTPSEPRLYDVWLMWVEFPDHPGIGKPRPVVITEVDGDLVSGIVAKITGNTDWDEAGDVPLLDWKAEGLLKPSLVRCS
QRFYFNRSELLQWFGRLSLRDAEHVNDGLEATLDIPPYRRSV

Download         Length: 369 bp

>T45445 NZ_CP006713:1118536-1118904 [Bifidobacterium breve JCM 7019]
ATGACCAGTACGCCAAGTGAACCGCGCCTGTATGACGTGTGGCTGATGTGGGTCGAGTTTCCCGACCATCCCGGTATCGG
CAAGCCGCGTCCCGTGGTAATCACCGAGGTTGACGGTGATCTGGTGTCGGGTATCGTGGCGAAGATAACCGGCAACACTG
ATTGGGATGAGGCCGGTGACGTGCCGCTGCTCGACTGGAAGGCCGAGGGGCTGTTGAAGCCGTCACTCGTGCGCTGTTCG
CAACGCTTCTACTTCAACAGGAGCGAACTGCTGCAATGGTTCGGACGACTCTCGTTGAGGGACGCGGAGCATGTTAACGA
CGGATTGGAAGCCACGTTGGACATTCCACCATACAGGCGGAGCGTATAG

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10516.81 Da        Isoelectric Point: 4.7309

>AT45445 WP_025221597.1 NZ_CP006713:1118271-1118555 [Bifidobacterium breve JCM 7019]
MGKLVANIDDDVKARAAALYDSMGMSLSTAVNMFLRQSLVDNGLPFKPTRHTPDGYPVPPVHNAYMFERSEKGHVILPAD
WNDSEDDVYDQYAK

Download         Length: 285 bp

>AT45445 NZ_CP006713:1118271-1118555 [Bifidobacterium breve JCM 7019]
ATGGGTAAGCTGGTCGCCAATATCGATGATGATGTCAAGGCGCGTGCCGCCGCGCTCTACGATTCCATGGGCATGAGCCT
GAGCACCGCAGTCAACATGTTTTTACGCCAGTCTCTGGTGGACAACGGGTTGCCGTTCAAGCCGACGCGGCACACGCCGG
ACGGCTATCCGGTGCCGCCTGTTCACAATGCCTACATGTTCGAGCGTTCGGAGAAGGGCCATGTGATACTGCCCGCCGAT
TGGAATGATTCGGAGGATGATGTCTATGACCAGTACGCCAAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB W6EYF0

Antitoxin

Source ID Structure
AlphaFold DB A0A0L7B6S4

References