Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1054890..1055526 Replicon chromosome
Accession NZ_CP006713
Organism Bifidobacterium breve JCM 7019

Toxin (Protein)


Gene name mazF Uniprot ID W6EZW1
Locus tag B7019_RS04445 Protein ID WP_014484977.1
Coordinates 1054890..1055246 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A2K9C8D0
Locus tag B7019_RS04450 Protein ID WP_025221547.1
Coordinates 1055233..1055526 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7019_RS04415 1049944..1050855 + 912 WP_025221542.1 ABC transporter ATP-binding protein -
B7019_RS04420 1051052..1051678 + 627 WP_003829226.1 30S ribosomal protein S4 -
B7019_RS04425 1052153..1052935 - 783 WP_025221543.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
B7019_RS04430 1053073..1053558 - 486 WP_025221544.1 cytidine deaminase -
B7019_RS04435 1053649..1054005 + 357 WP_025221545.1 SdpI family protein -
B7019_RS04440 1054110..1054712 + 603 WP_025221546.1 transglutaminase family protein -
B7019_RS04445 1054890..1055246 - 357 WP_014484977.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
B7019_RS04450 1055233..1055526 - 294 WP_025221547.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
B7019_RS04455 1055778..1056452 + 675 WP_025221548.1 histidine phosphatase family protein -
B7019_RS04460 1056535..1056918 + 384 WP_016463075.1 DUF948 domain-containing protein -
B7019_RS04465 1056918..1057154 + 237 WP_025221549.1 hypothetical protein -
B7019_RS04470 1057250..1059928 + 2679 WP_025221550.1 alanine--tRNA ligase -
B7019_RS04475 1059937..1060389 + 453 WP_003829241.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-111)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13302.25 Da        Isoelectric Point: 8.9603

>T45444 WP_014484977.1 NZ_CP006713:c1055246-1054890 [Bifidobacterium breve JCM 7019]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVAWAINELYPGLLA

Download         Length: 357 bp

>T45444 NZ_CP006713:c1055246-1054890 [Bifidobacterium breve JCM 7019]
ATGATGAAGACTGATCCGCGCCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAATCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCGCCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10836.28 Da        Isoelectric Point: 4.2896

>AT45444 WP_025221547.1 NZ_CP006713:c1055526-1055233 [Bifidobacterium breve JCM 7019]
MAIKEKATISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT45444 NZ_CP006713:c1055526-1055233 [Bifidobacterium breve JCM 7019]
ATGGCTATCAAGGAGAAGGCCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7D489

Antitoxin

Source ID Structure
AlphaFold DB A0A2K9C8D0

References