Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1052282..1052918 Replicon chromosome
Accession NZ_CP006712
Organism Bifidobacterium breve JCM 7017

Toxin (Protein)


Gene name mazF Uniprot ID D4BP71
Locus tag B7017_RS04325 Protein ID WP_003829234.1
Coordinates 1052282..1052638 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID D6PAX1
Locus tag B7017_RS04330 Protein ID WP_012577921.1
Coordinates 1052625..1052918 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7017_RS04295 1047474..1048385 + 912 WP_003829224.1 ABC transporter ATP-binding protein -
B7017_RS04300 1048582..1049208 + 627 WP_003829226.1 30S ribosomal protein S4 -
B7017_RS04305 1049545..1050327 - 783 WP_025301204.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
B7017_RS04310 1050467..1050952 - 486 WP_003829229.1 cytidine deaminase -
B7017_RS04315 1051042..1051398 + 357 WP_003833231.1 SdpI family protein -
B7017_RS04320 1051503..1052105 + 603 WP_003829233.1 transglutaminase family protein -
B7017_RS04325 1052282..1052638 - 357 WP_003829234.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
B7017_RS04330 1052625..1052918 - 294 WP_012577921.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
B7017_RS04335 1053069..1053233 - 165 Protein_850 IS30 family transposase -
B7017_RS04340 1053333..1054007 + 675 WP_025301205.1 histidine phosphatase family protein -
B7017_RS04345 1054090..1054473 + 384 WP_025301206.1 DUF948 domain-containing protein -
B7017_RS04350 1054473..1054709 + 237 WP_003829239.1 hypothetical protein -
B7017_RS04355 1054805..1057483 + 2679 WP_003829240.1 alanine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-110)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13316.23 Da        Isoelectric Point: 7.9984

>T45442 WP_003829234.1 NZ_CP006712:c1052638-1052282 [Bifidobacterium breve JCM 7017]
MMKTDPHQFEIWWVPFTFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSSLFLDDGPTGRLSAYDAGRVTWAINELYPGLLA

Download         Length: 357 bp

>T45442 NZ_CP006712:c1052638-1052282 [Bifidobacterium breve JCM 7017]
ATGATGAAGACTGATCCGCACCAGTTCGAGATATGGTGGGTGCCGTTCACTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAGTCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCACCTG
GGCGATCAACGAACTCTATCCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10866.31 Da        Isoelectric Point: 4.2896

>AT45442 WP_012577921.1 NZ_CP006712:c1052918-1052625 [Bifidobacterium breve JCM 7017]
MAIKEKTTISLDAQTKRDGIAILDAMGLNLSTFAEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT45442 NZ_CP006712:c1052918-1052625 [Bifidobacterium breve JCM 7017]
ATGGCTATCAAGGAGAAGACCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGCCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7B6G7

Antitoxin

Source ID Structure
AlphaFold DB A0A1S2VTZ6

References