Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 992887..993442 Replicon chromosome
Accession NZ_CP006712
Organism Bifidobacterium breve JCM 7017

Toxin (Protein)


Gene name mazF Uniprot ID A0A0L7C7B1
Locus tag B7017_RS04055 Protein ID WP_025301183.1
Coordinates 992887..993210 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag B7017_RS04060 Protein ID WP_003829112.1
Coordinates 993197..993442 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B7017_RS04030 987915..988213 - 299 Protein_787 IS256 family transposase -
B7017_RS04035 988683..989948 - 1266 WP_015438706.1 Fic family protein -
B7017_RS04040 990490..990855 - 366 WP_025299744.1 YccF domain-containing protein -
B7017_RS04045 991093..991962 + 870 WP_003829104.1 class I SAM-dependent methyltransferase -
B7017_RS09915 992056..992670 + 615 WP_025301182.1 cupin domain-containing protein -
B7017_RS04055 992887..993210 - 324 WP_025301183.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
B7017_RS04060 993197..993442 - 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
B7017_RS04065 993614..994870 + 1257 WP_015438708.1 HipA domain-containing protein -
B7017_RS04070 995136..995327 + 192 WP_003829118.1 hypothetical protein -
B7017_RS04075 995511..996782 - 1272 WP_025301184.1 MFS transporter -
B7017_RS04080 996939..997601 - 663 WP_014483844.1 TetR/AcrR family transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 983558..1030954 47396


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-106)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11887.72 Da        Isoelectric Point: 5.0771

>T45441 WP_025301183.1 NZ_CP006712:c993210-992887 [Bifidobacterium breve JCM 7017]
MKRGEIWTLQADGYVSKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T45441 NZ_CP006712:c993210-992887 [Bifidobacterium breve JCM 7017]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGTCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT45441 WP_003829112.1 NZ_CP006712:c993442-993197 [Bifidobacterium breve JCM 7017]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT45441 NZ_CP006712:c993442-993197 [Bifidobacterium breve JCM 7017]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCAGGCAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0L7C7B1

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References