Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/RelB(antitoxin)
Location 1014688..1015324 Replicon chromosome
Accession NZ_CP006711
Organism Bifidobacterium breve 12L

Toxin (Protein)


Gene name mazF Uniprot ID -
Locus tag B12L_RS04170 Protein ID WP_025299776.1
Coordinates 1014688..1015044 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID -
Locus tag B12L_RS04175 Protein ID WP_025299777.1
Coordinates 1015031..1015324 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B12L_RS04140 1009741..1010652 + 912 WP_025299772.1 ABC transporter ATP-binding protein -
B12L_RS04145 1010849..1011475 + 627 WP_003829226.1 30S ribosomal protein S4 -
B12L_RS04150 1011950..1012732 - 783 WP_025299773.1 gamma-glutamyl-gamma-aminobutyrate hydrolase family protein -
B12L_RS04155 1012870..1013355 - 486 WP_025299774.1 cytidine deaminase -
B12L_RS04160 1013448..1013804 + 357 WP_025299775.1 SdpI family protein -
B12L_RS04165 1013909..1014511 + 603 WP_003829233.1 transglutaminase family protein -
B12L_RS04170 1014688..1015044 - 357 WP_025299776.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
B12L_RS04175 1015031..1015324 - 294 WP_025299777.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
B12L_RS04180 1015576..1016250 + 675 WP_015438748.1 histidine phosphatase family protein -
B12L_RS04185 1016333..1016716 + 384 WP_016463075.1 DUF948 domain-containing protein -
B12L_RS04190 1016716..1016952 + 237 WP_003829239.1 hypothetical protein -
B12L_RS04195 1017048..1019726 + 2679 WP_025299778.1 alanine--tRNA ligase -
B12L_RS04200 1019735..1020187 + 453 WP_003829241.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(7-111)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(11-53)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 13292.21 Da        Isoelectric Point: 8.9603

>T45440 WP_025299776.1 NZ_CP006711:c1015044-1014688 [Bifidobacterium breve 12L]
MMKTDPRQFEIWWVPFAFPDKPGKAKNRPSVILQWDDGTRIALVTKVTGNTWRDEPGYVVLRDWQDAGLSKPSAVRCSQL
LRLPSNLFLDDGPTGRLSAYDAGRVAWAINELYSGLLA

Download         Length: 357 bp

>T45440 NZ_CP006711:c1015044-1014688 [Bifidobacterium breve 12L]
ATGATGAAGACTGATCCGCGCCAGTTCGAGATATGGTGGGTGCCGTTCGCTTTTCCCGACAAGCCAGGCAAAGCGAAGAA
CCGTCCCAGCGTGATCCTCCAATGGGACGACGGCACCCGCATCGCCTTGGTGACCAAGGTCACCGGCAACACCTGGCGCG
ACGAACCGGGCTATGTGGTGCTGCGTGACTGGCAGGACGCGGGACTGAGCAAGCCCAGCGCCGTGCGATGCTCGCAACTG
CTGCGGCTGCCAAGCAATCTTTTCCTCGACGACGGCCCGACAGGAAGACTTTCGGCATATGATGCCGGCCGCGTCGCCTG
GGCGATCAACGAACTCTATTCGGGCCTACTAGCCTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 10864.33 Da        Isoelectric Point: 4.2896

>AT45440 WP_025299777.1 NZ_CP006711:c1015324-1015031 [Bifidobacterium breve 12L]
MAIKEKATISLDAQTKRDGIAILDAMGLNLSTFVEMSLRQLVRDGRLPFTPSVRPSFEKDNEGYPLFKANMDDPRIVTPQ
IRDGAVILPEGWDDDED

Download         Length: 294 bp

>AT45440 NZ_CP006711:c1015324-1015031 [Bifidobacterium breve 12L]
ATGGCTATCAAGGAGAAGGCCACGATCAGTCTGGATGCGCAGACCAAGCGTGACGGCATCGCCATACTTGACGCCATGGG
GTTGAACCTGAGCACGTTTGTCGAAATGTCTCTCAGACAATTGGTACGCGACGGGCGTCTGCCATTCACGCCAAGCGTCC
GCCCTTCATTCGAAAAAGACAACGAGGGATATCCCCTGTTCAAAGCGAACATGGATGATCCCCGCATCGTGACGCCCCAG
ATACGTGATGGTGCCGTGATCCTGCCGGAGGGTTGGGACGATGATGAAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References