Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PemK-RelB
Location 954436..954991 Replicon chromosome
Accession NZ_CP006711
Organism Bifidobacterium breve 12L

Toxin (Protein)


Gene name mazF Uniprot ID S2ZXD7
Locus tag B12L_RS03890 Protein ID WP_003829109.1
Coordinates 954436..954759 (-) Length 108 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID S2ZDC4
Locus tag B12L_RS03895 Protein ID WP_003829112.1
Coordinates 954746..954991 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B12L_RS09745 949468..949756 - 289 Protein_759 IS256 family transposase -
B12L_RS03870 950232..951497 - 1266 WP_025299743.1 Fic family protein -
B12L_RS03875 952039..952404 - 366 WP_025299744.1 YccF domain-containing protein -
B12L_RS03880 952642..953511 + 870 WP_003829104.1 class I SAM-dependent methyltransferase -
B12L_RS09755 953605..954219 + 615 WP_003829107.1 cupin domain-containing protein -
B12L_RS03890 954436..954759 - 324 WP_003829109.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
B12L_RS03895 954746..954991 - 246 WP_003829112.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
B12L_RS03900 955163..956419 + 1257 WP_019728042.1 HipA domain-containing protein -
B12L_RS03905 956684..956875 + 192 WP_003829118.1 hypothetical protein -
B12L_RS03910 957059..958330 - 1272 WP_003829121.1 MFS transporter -
B12L_RS03915 958487..959149 - 663 WP_019728044.1 TetR/AcrR family transcriptional regulator -
B12L_RS10375 959612..959797 + 186 WP_003808830.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 945117..979600 34483


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-103)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-56)


Sequences

Toxin        


Download         Length: 108 a.a.        Molecular weight: 11859.67 Da        Isoelectric Point: 5.0771

>T45439 WP_003829109.1 NZ_CP006711:c954759-954436 [Bifidobacterium breve 12L]
MKRGEIWTLQADGYASKPRPVVIVQSDAVDRFDSVITCLLTSYDSSDIDTRVRLEPSPENGLNKVSYVMTDKIVTVDRKL
LGYQVGVVDDAAMANIGRQLMRVLGLL

Download         Length: 324 bp

>T45439 NZ_CP006711:c954759-954436 [Bifidobacterium breve 12L]
ATGAAACGAGGTGAGATTTGGACGTTGCAGGCCGATGGCTACGCCAGCAAGCCACGGCCGGTTGTCATCGTGCAGAGCGA
TGCCGTCGATCGGTTCGATTCCGTCATCACTTGTCTGCTGACCTCATACGATTCCTCCGACATCGACACGCGGGTCCGAT
TGGAACCAAGTCCGGAGAACGGCCTGAACAAAGTGAGTTACGTGATGACGGACAAGATCGTCACCGTCGACCGCAAACTG
CTGGGATACCAAGTCGGCGTGGTTGATGATGCAGCCATGGCGAATATCGGCCGTCAGCTCATGCGGGTTCTAGGTTTGCT
GTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9339.43 Da        Isoelectric Point: 4.5881

>AT45439 WP_003829112.1 NZ_CP006711:c954991-954746 [Bifidobacterium breve 12L]
MSMQIATRVDDEQAALFKETTRQLGTTPADALRMFISAFNDYRGFPYEVRLPRNDVEPFASERDATEYASRLALRMSDET
R

Download         Length: 246 bp

>AT45439 NZ_CP006711:c954991-954746 [Bifidobacterium breve 12L]
ATGAGCATGCAGATTGCCACGCGAGTTGATGACGAGCAGGCTGCGTTGTTTAAGGAGACGACCCGACAGCTGGGTACCAC
CCCGGCCGATGCCCTGCGTATGTTCATCAGCGCGTTCAATGATTACCGTGGGTTCCCCTATGAGGTGCGTTTGCCACGAA
ATGACGTGGAGCCGTTTGCGTCCGAGCGGGACGCTACTGAGTATGCCAGTCGTCTCGCCTTGAGGATGTCCGATGAAACG
AGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A087B6Q7

Antitoxin

Source ID Structure
AlphaFold DB A0A087B6Q6

References