Detailed information of TA system TA045437

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 3093683..3094284
• Replicon: chromosome
• Accession: NZ_CP006704
• Organism: Comamonas testosteroni TK102

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: O987_RS13975
• Protein ID: WP_019042333.1
• Coordinates: 3093683..3094069 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A076PMR1
• Locus tag: O987_RS13980
• Protein ID: WP_034356765.1
• Coordinates: 3094069..3094284 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
O987_RS13960	3091150..3092460	-	1311	WP_034356769.1	DNA polymerase Y family protein	-
O987_RS13965	3092405..3093205	-	801	WP_122974731.1	translesion DNA synthesis-associated protein ImuA	-
O987_RS13970	3093385..3093594	-	210	WP_034356767.1	hypothetical protein	-
O987_RS13975	3093683..3094069	-	387	WP_019042333.1	twitching motility protein PilT	Toxin
O987_RS13980	3094069..3094284	-	216	WP_034356765.1	DUF2191 domain-containing protein	Antitoxin
O987_RS13985	3094369..3095186	+	818	Protein_2757	SprT-like domain-containing protein	-
O987_RS27585	3095340..3095666	+	327	WP_019042331.1	transposase	-
O987_RS28070	3096009..3096914	-	906	Protein_2759	phosphoethanolamine transferase	-
O987_RS28075	3097928..3098317	+	390	WP_080571250.1	CzcE family metal-binding protein	-
O987_RS28080	3098397..3098843	-	447	WP_080731523.1	Cd(II)/Pb(II)-responsive transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Type I toxin-antitoxin system, toxin Ldr (1-35)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 13999.29 Da
• Isoelectric Point: 5.0594

>T45437 WP_019042333.1 NZ_CP006704:c3094069-3093683 [Comamonas testosteroni TK102]
MPVSVLVDTSVWVRHFREADTHLQELLMQDSVLMHSMVHAELACGTPPAPRADTLAALAMLRPSQEASIPETLEFLEQNK
LFGKGCGLVDLSLLASTLITPGALLWTADRRLADMATQLNVAYLPPLH

Nucleotide

• Length: 387 bp

>T45437 NZ_CP006704:c3094069-3093683 [Comamonas testosteroni TK102]
ATGCCCGTGTCCGTCCTAGTCGATACATCAGTGTGGGTGAGACATTTTCGTGAGGCAGACACTCACCTGCAGGAATTGCT
TATGCAAGACAGCGTGCTGATGCATTCCATGGTGCATGCAGAGCTGGCCTGTGGTACGCCGCCAGCACCGCGTGCCGACA
CGCTGGCGGCTCTGGCGATGCTGCGGCCGAGCCAGGAGGCATCAATTCCAGAGACTTTGGAATTTCTGGAGCAGAACAAG
CTTTTCGGCAAGGGCTGCGGCTTAGTAGATCTATCGCTGCTTGCCTCTACGCTTATCACTCCTGGCGCCTTACTTTGGAC
TGCAGATCGACGCCTCGCCGACATGGCCACACAGTTGAACGTGGCCTATCTACCGCCTTTGCATTGA

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7519.50 Da
• Isoelectric Point: 5.0166

>AT45437 WP_034356765.1 NZ_CP006704:c3094284-3094069 [Comamonas testosteroni TK102]
MRSTFTIDDDVVNRARAVAAPGIAVPELVRLALETFTRVEAGKRLAALGGAAPNMPDVPRRGSEADAEDSR

Nucleotide

• Length: 216 bp

>AT45437 NZ_CP006704:c3094284-3094069 [Comamonas testosteroni TK102]
ATGAGATCGACTTTCACGATCGATGACGATGTCGTAAACCGCGCACGCGCCGTCGCGGCACCCGGTATTGCTGTGCCGGA
ACTGGTACGACTCGCACTGGAAACCTTCACACGTGTCGAGGCAGGTAAGCGTTTGGCTGCGCTCGGCGGTGCCGCGCCAA
ATATGCCCGATGTGCCGCGCCGTGGCAGTGAGGCGGATGCTGAGGACTCACGCTAA

Structures

Antitoxin

• AlphaFold DB: A0A076PMR1