Detailed information of TA system TA045436

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tad-ata/COG5606-COG4679
• Location: 17207..17877
• Replicon: plasmid unnamed1
• Accession: NZ_CP006697
• Organism: Xylella fastidiosa subsp. sandyi Ann-1

Toxin (Protein)

• Gene name: tad
• Uniprot ID: -
• Locus tag: D934_RS12830
• Protein ID: WP_020852254.1
• Coordinates: 17207..17557 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: ata
• Uniprot ID: -
• Locus tag: D934_RS12835
• Protein ID: WP_020852255.1
• Coordinates: 17554..17877 (+)
• Length: 108 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D934_RS12800	12791..13243	-	453	WP_051404191.1	type II toxin-antitoxin system VapC family toxin	-
D934_RS12805	13243..13497	-	255	WP_024748605.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
D934_RS12810	13882..14111	+	230	Protein_21	recombinase family protein	-
D934_RS12815	14480..15961	+	1482	WP_024748606.1	D-alanyl-D-alanine carboxypeptidase/D-alanyl-D-alanine-endopeptidase	-
D934_RS14905	16534..16710	+	177	WP_196230051.1	hypothetical protein	-
D934_RS12825	16714..17134	+	421	Protein_24	virulence factor	-
D934_RS12830	17207..17557	+	351	WP_020852254.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D934_RS12835	17554..17877	+	324	WP_020852255.1	helix-turn-helix domain-containing protein	Antitoxin
D934_RS12850	18590..18790	+	201	WP_024748608.1	hypothetical protein	-
D934_RS12855	18787..19272	+	486	WP_024748609.1	hypothetical protein	-
D934_RS12860	19275..19595	+	321	WP_020852257.1	TrbC/VirB2 family protein	-
D934_RS12865	19600..19983	+	384	WP_024748610.1	VirB3 family type IV secretion system protein	-
D934_RS12870	19946..22360	+	2415	WP_024748611.1	VirB4 family type IV secretion system protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..30305	30305

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-105)

Antitoxin

• Cro/C1-type helix-turn-helix domain (32-82)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12745.60 Da
• Isoelectric Point: 8.8688

>T45436 WP_020852254.1 NZ_CP006697:17207-17557 [Xylella fastidiosa subsp. sandyi Ann-1]
MKPLVWIASAKKDLMAMPDDVQDIFGYALHLAQTGEKHDQAKSLKGFGSAGVLEVVEDSDGGTYRGVYTVRFGNAVYVLH
CFQKKSTHGIATSKPDIDLIRDRLKVAEAHAKGERK

Nucleotide

• Length: 351 bp

>T45436 NZ_CP006697:17207-17557 [Xylella fastidiosa subsp. sandyi Ann-1]
ATGAAACCGTTAGTTTGGATCGCTTCGGCCAAAAAAGACCTGATGGCGATGCCGGACGACGTACAGGACATTTTCGGGTA
CGCGCTGCATTTAGCTCAGACCGGCGAGAAGCACGATCAGGCCAAGTCGCTCAAAGGATTCGGTTCGGCTGGCGTGCTCG
AAGTGGTCGAGGATTCAGACGGCGGCACATACCGCGGTGTCTACACGGTGCGATTCGGAAACGCGGTTTATGTCCTGCAT
TGCTTCCAGAAGAAATCGACGCATGGCATAGCGACGTCAAAGCCGGATATTGACCTCATTCGAGATCGATTGAAGGTCGC
CGAGGCGCACGCGAAAGGAGAACGGAAATGA

Antitoxin

Protein

• Length: 108 a.a.
• Molecular weight: 11635.52 Da
• Isoelectric Point: 5.8625

>AT45436 WP_020852255.1 NZ_CP006697:17554-17877 [Xylella fastidiosa subsp. sandyi Ann-1]
MIEIEEGSGNVYADLGIPDADEMLVKAQLATKIGEIIKGRGWTQQEAADVLGMTQPKLSKMLRGQFRGISEAKMLECLAR
LGRQVQIVVGPARRTNDVGHVEVIFAA

Nucleotide

• Length: 324 bp

>AT45436 NZ_CP006697:17554-17877 [Xylella fastidiosa subsp. sandyi Ann-1]
ATGATCGAAATTGAAGAAGGTAGCGGCAACGTCTATGCCGACCTTGGCATTCCTGACGCCGACGAAATGCTGGTGAAGGC
GCAGCTTGCCACGAAGATCGGCGAGATCATCAAGGGTCGCGGATGGACTCAGCAAGAAGCAGCCGACGTGCTCGGCATGA
CACAACCCAAGCTGTCGAAGATGCTGCGCGGACAGTTCCGAGGTATCAGCGAAGCGAAGATGCTGGAATGCCTCGCGCGG
CTTGGCCGGCAAGTTCAGATCGTTGTCGGTCCCGCTCGCCGTACAAACGACGTGGGGCACGTCGAAGTCATATTCGCGGC
CTAA