Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/PRK09812-MazE
Location 2750..3336 Replicon plasmid unnamed1
Accession NZ_CP006697
Organism Xylella fastidiosa subsp. sandyi Ann-1

Toxin (Protein)


Gene name pemK Uniprot ID A0A060HFH6
Locus tag D934_RS12725 Protein ID WP_024748613.1
Coordinates 2750..3076 (-) Length 109 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID D5LL99
Locus tag D934_RS12730 Protein ID WP_013087943.1
Coordinates 3073..3336 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
D934_RS12710 74..1180 + 1107 WP_020852265.1 hypothetical protein -
D934_RS12715 1228..1515 + 288 WP_020852266.1 DUF4190 domain-containing protein -
D934_RS12720 1687..2691 + 1005 WP_020852267.1 relaxase/mobilization nuclease domain-containing protein -
D934_RS12725 2750..3076 - 327 WP_024748613.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
D934_RS12730 3073..3336 - 264 WP_013087943.1 antitoxin Antitoxin
D934_RS12735 3448..4656 + 1209 WP_042836803.1 transposase -
D934_RS12740 4936..5199 + 264 WP_080702547.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
D934_RS12745 5171..5500 + 330 WP_024748578.1 type II toxin-antitoxin system YafQ family toxin -
D934_RS12750 5631..6074 - 444 WP_014607318.1 hypothetical protein -
D934_RS12755 6075..6827 - 753 WP_020852243.1 hypothetical protein -
D934_RS12760 6840..7196 - 357 WP_020852242.1 hypothetical protein -
D934_RS12765 7540..8103 + 564 WP_038273547.1 plasmid mobilization relaxosome protein MobC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..30305 30305


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(2-102)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 109 a.a.        Molecular weight: 11795.71 Da        Isoelectric Point: 8.5480

>T45435 WP_024748613.1 NZ_CP006697:c3076-2750 [Xylella fastidiosa subsp. sandyi Ann-1]
MKRGDVYMVDLEPTAGHEQRGHRPVVVISSERFNRLTSCPVILPITNGGEFASRLGFAVELLGTITTGVVRCDQPRALDL
IARNARKVESLPPNILADVLAKAVTIFQ

Download         Length: 327 bp

>T45435 NZ_CP006697:c3076-2750 [Xylella fastidiosa subsp. sandyi Ann-1]
ATGAAACGCGGCGATGTTTACATGGTCGATCTGGAGCCAACCGCCGGGCACGAACAACGCGGTCACCGTCCGGTGGTTGT
TATCTCCAGTGAGCGATTCAATCGGCTTACCTCTTGTCCGGTGATCCTTCCAATCACAAACGGCGGCGAGTTCGCCTCCC
GCCTCGGGTTCGCTGTGGAATTACTAGGTACGATTACAACGGGCGTCGTTCGATGTGATCAACCACGTGCACTCGACTTA
ATCGCTCGCAATGCCCGCAAAGTGGAAAGCCTACCACCAAACATCTTGGCCGATGTCTTGGCAAAAGCTGTCACCATCTT
CCAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9365.77 Da        Isoelectric Point: 4.3588

>AT45435 WP_013087943.1 NZ_CP006697:c3336-3073 [Xylella fastidiosa subsp. sandyi Ann-1]
MHTTNLRKVGGSIMLAVPPAFLDQLHLEVGATVGLAVTDGRLVIEPVLHPQYTLDQLLAEAEASGAYPLPPEEREWVDAP
AVGRELI

Download         Length: 264 bp

>AT45435 NZ_CP006697:c3336-3073 [Xylella fastidiosa subsp. sandyi Ann-1]
ATGCACACCACAAACCTTCGTAAAGTCGGCGGATCGATCATGCTGGCCGTTCCCCCCGCTTTCCTTGACCAGCTTCATCT
TGAGGTCGGCGCAACGGTCGGCTTAGCCGTCACAGACGGTCGTCTGGTCATTGAGCCAGTCCTACATCCTCAGTACACGC
TGGATCAACTGCTGGCCGAAGCGGAAGCCTCCGGGGCTTACCCGTTGCCGCCCGAGGAACGTGAATGGGTCGATGCTCCA
GCAGTAGGACGCGAACTGATATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060HFH6

Antitoxin

Source ID Structure
AlphaFold DB A0A060H856

References