Detailed information of TA system TA045431

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 2150078..2150664
• Replicon: chromosome
• Accession: NZ_CP006696
• Organism: Xylella fastidiosa subsp. sandyi Ann-1

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: D934_RS13930
• Protein ID: WP_080702508.1
• Coordinates: 2150410..2150664 (-)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: B2I6C9
• Locus tag: D934_RS10040
• Protein ID: WP_004085025.1
• Coordinates: 2150078..2150413 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D934_RS10010	2146032..2146916	-	885	WP_051404211.1	iron-sulfur cluster carrier protein ApbC	-
D934_RS10015	2147381..2148154	+	774	WP_020852623.1	3-deoxy-manno-octulosonate cytidylyltransferase	-
D934_RS10020	2148151..2148633	+	483	WP_020852624.1	low molecular weight phosphotyrosine protein phosphatase	-
D934_RS10025	2148611..2148904	-	294	Protein_1962	DNA modification methylase	-
D934_RS10030	2149307..2149588	+	282	WP_020852626.1	type II toxin-antitoxin system RelE/ParE family toxin	-
D934_RS10035	2149599..2149898	+	300	WP_020852627.1	HigA family addiction module antidote protein	-
D934_RS10040	2150078..2150413	-	336	WP_004085025.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
D934_RS13930	2150410..2150664	-	255	WP_080702508.1	type II toxin-antitoxin system HicA family toxin	Toxin
D934_RS10045	2150717..2151415	-	699	WP_020852371.1	phage-related protein	-
D934_RS10050	2151544..2152001	-	458	Protein_1968	hypothetical protein	-
D934_RS10055	2151998..2152762	-	765	WP_020852629.1	hypothetical protein	-
D934_RS10060	2152668..2153650	-	983	Protein_1970	DUF1376 domain-containing protein	-
D934_RS10065	2153752..2154342	+	591	WP_020852632.1	recombinase family protein	-
D934_RS10070	2154399..2155187	-	789	WP_020852633.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2132005..2154342	22337

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-80)

Antitoxin

• Uncharacterised protein family HicB (55-105)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9661.19 Da
• Isoelectric Point: 11.5325

>T45431 WP_080702508.1 NZ_CP006696:c2150664-2150410 [Xylella fastidiosa subsp. sandyi Ann-1]
MKRKHAKTLSAIYTRPVSANIQWRHIEALFVELGAKVEEREGSRMLVRLFGERRIFHRPHPSPNTDKGAVESIRAWLKSN
GVTP

Nucleotide

• Length: 255 bp

>T45431 NZ_CP006696:c2150664-2150410 [Xylella fastidiosa subsp. sandyi Ann-1]
ATGAAGCGTAAGCACGCTAAGACTCTCAGCGCTATATACACACGCCCTGTTTCGGCCAACATTCAATGGCGCCATATTGA
AGCGCTGTTCGTGGAGCTTGGAGCGAAAGTAGAAGAACGTGAAGGTTCTCGTATGTTGGTGCGGCTGTTCGGTGAACGTC
GGATATTCCACCGGCCCCACCCTTCGCCTAACACTGACAAGGGTGCTGTTGAATCAATCCGAGCTTGGTTGAAATCGAAC
GGAGTTACACCATGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12324.07 Da
• Isoelectric Point: 5.6556

>AT45431 WP_004085025.1 NZ_CP006696:c2150413-2150078 [Xylella fastidiosa subsp. sandyi Ann-1]
MKNIMTIESFKAIITYDPEIDMFRGEFVGINGGADFYAKDLKGLRREGAISLKVFLEACKEDSVEPRKCYSGKFNARISP
ELHALASEAAAAQGISLNQFVEQAIQHEVHT

Nucleotide

• Length: 336 bp

>AT45431 NZ_CP006696:c2150413-2150078 [Xylella fastidiosa subsp. sandyi Ann-1]
ATGAAGAACATCATGACTATTGAGAGCTTTAAAGCCATCATTACCTATGATCCGGAGATCGACATGTTTCGCGGTGAATT
TGTGGGTATCAATGGCGGTGCTGATTTCTATGCAAAAGACCTTAAGGGTCTTCGTCGAGAAGGAGCTATTTCTCTGAAGG
TATTTTTGGAAGCCTGCAAAGAGGATAGCGTGGAACCACGTAAATGCTACTCAGGGAAGTTCAATGCAAGGATTTCTCCT
GAACTGCATGCTTTGGCTTCTGAAGCCGCAGCAGCACAGGGCATCAGTTTGAACCAATTTGTAGAACAAGCGATTCAGCA
TGAAGTCCACACCTGA

Structures

Antitoxin

• AlphaFold DB: A0A060HAK5