Detailed information of TA system TA045430

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK-MazE
• Location: 1833295..1833836
• Replicon: chromosome
• Accession: NZ_CP006696
• Organism: Xylella fastidiosa subsp. sandyi Ann-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: D934_RS08605
• Protein ID: WP_020853079.1
• Coordinates: 1833295..1833618 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: D934_RS08610
• Protein ID: WP_024748714.1
• Coordinates: 1833615..1833836 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D934_RS13695	1828414..1829244	+	831	WP_080702484.1	site-specific integrase	-
D934_RS08585	1829965..1830348	-	384	WP_051604010.1	hypothetical protein	-
D934_RS08590	1830491..1830784	+	294	WP_024748833.1	hypothetical protein	-
D934_RS13700	1830786..1831052	+	267	WP_080702485.1	hypothetical protein	-
D934_RS13705	1831056..1831238	+	183	WP_020853077.1	hypothetical protein	-
D934_RS08595	1831612..1832835	+	1224	WP_051604011.1	replication initiation factor domain-containing protein	-
D934_RS08600	1832822..1833154	+	333	WP_020853080.1	hypothetical protein	-
D934_RS08605	1833295..1833618	-	324	WP_020853079.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
D934_RS08610	1833615..1833836	-	222	WP_024748714.1	antitoxin MazE family protein	Antitoxin
D934_RS08615	1834160..1834537	-	378	WP_024748713.1	DUF3693 domain-containing protein	-
D934_RS08620	1834675..1834977	+	303	WP_042836558.1	hypothetical protein	-
D934_RS13715	1834970..1835236	+	267	WP_080702486.1	hypothetical protein	-
D934_RS13720	1835240..1835422	+	183	WP_020853101.1	hypothetical protein	-
D934_RS08630	1835920..1837095	+	1176	WP_020853102.1	replication initiation factor domain-containing protein	-
D934_RS08635	1837140..1837451	+	312	WP_042836560.1	hypothetical protein	-
D934_RS08640	1837463..1837666	+	204	WP_042836563.1	hypothetical protein	-
D934_RS08645	1837675..1837908	+	234	WP_024749195.1	phage coat protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1785029..1858374	73345

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-105)

Antitoxin

• Antitoxin MazE-like (9-72)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 11808.87 Da
• Isoelectric Point: 7.9848

>T45430 WP_020853079.1 NZ_CP006696:c1833618-1833295 [Xylella fastidiosa subsp. sandyi Ann-1]
MIQRGDLVAVSLQGDYGKPRPALIVQSDLLTELDSVVLCPVTSDLRNAIFRVTVEPTPANGLRTLSQVMVDKISTLPRNK
INEPFGRINDERMKAIERALLLIVGII

Nucleotide

• Length: 324 bp

>T45430 NZ_CP006696:c1833618-1833295 [Xylella fastidiosa subsp. sandyi Ann-1]
ATGATTCAACGTGGCGACCTTGTGGCAGTTTCCCTACAAGGCGACTACGGCAAGCCTAGACCAGCGTTGATCGTTCAATC
AGACCTTCTAACAGAGTTGGATAGTGTCGTACTGTGTCCGGTCACAAGCGACCTGCGGAATGCGATATTTCGTGTCACCG
TTGAACCGACACCGGCCAACGGTTTACGGACGCTATCGCAGGTCATGGTAGATAAAATCTCAACACTGCCACGTAACAAA
ATCAACGAGCCTTTTGGACGTATCAATGATGAGAGGATGAAAGCAATAGAGAGAGCATTGTTACTGATCGTTGGTATTAT
TTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8507.76 Da
• Isoelectric Point: 10.3534

>AT45430 WP_024748714.1 NZ_CP006696:c1833836-1833615 [Xylella fastidiosa subsp. sandyi Ann-1]
MTSIHRPDSKVVHHRKQMRAAGLRPVQFWVPDTRTPEFAAEIRSQCRALKSEQAEADVLRFTEKAITYIEGWK

Nucleotide

• Length: 222 bp

>AT45430 NZ_CP006696:c1833836-1833615 [Xylella fastidiosa subsp. sandyi Ann-1]
ATGACATCAATTCACAGACCAGATAGCAAGGTAGTTCACCACAGGAAACAAATGCGCGCAGCGGGATTGCGTCCTGTGCA
GTTCTGGGTACCAGATACGCGCACACCAGAATTCGCTGCTGAAATTCGAAGCCAGTGCCGCGCCCTGAAGAGCGAACAGG
CAGAAGCCGACGTTTTACGCTTCACAGAAAAAGCCATCACCTATATTGAAGGTTGGAAATGA