Detailed information of TA system TA045429

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-DUF971
• Location: 1724234..1724839
• Replicon: chromosome
• Accession: NZ_CP006696
• Organism: Xylella fastidiosa subsp. sandyi Ann-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: D934_RS08085
• Protein ID: WP_004089252.1
• Coordinates: 1724543..1724839 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: D934_RS08080
• Protein ID: WP_004089254.1
• Coordinates: 1724234..1724539 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D934_RS08045	1720488..1720892	-	405	WP_024748528.1	type II toxin-antitoxin system VapC family toxin	-
D934_RS08050	1720889..1721161	-	273	WP_024748527.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
D934_RS08055	1721321..1722091	+	771	WP_020851656.1	hypothetical protein	-
D934_RS08060	1722091..1722408	+	318	WP_020851655.1	hypothetical protein	-
D934_RS08065	1722405..1723235	+	831	WP_020851654.1	hypothetical protein	-
D934_RS08070	1723232..1723873	+	642	WP_020851653.1	phage-related protein	-
D934_RS08075	1723870..1724223	+	354	WP_020851652.1	hypothetical protein	-
D934_RS08080	1724234..1724539	-	306	WP_004089254.1	putative addiction module antidote protein	Antitoxin
D934_RS08085	1724543..1724839	-	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D934_RS08090	1724938..1726083	+	1146	WP_020851651.1	baseplate J/gp47 family protein	-
D934_RS08095	1726080..1726640	+	561	WP_020851650.1	DUF2612 domain-containing protein	-
D934_RS08100	1726644..1727807	+	1164	WP_020851649.1	phage tail protein	-
D934_RS08105	1727946..1728386	-	441	WP_024749309.1	helix-turn-helix domain-containing protein	-
D934_RS14475	1728389..1728595	-	207	WP_155244291.1	DUF4258 domain-containing protein	-
D934_RS08110	1729190..1729383	+	194	Protein_1572	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1675562..1727807	52245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T45429 WP_004089252.1 NZ_CP006696:c1724839-1724543 [Xylella fastidiosa subsp. sandyi Ann-1]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T45429 NZ_CP006696:c1724839-1724543 [Xylella fastidiosa subsp. sandyi Ann-1]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATATCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10983.37 Da
• Isoelectric Point: 5.0941

>AT45429 WP_004089254.1 NZ_CP006696:c1724539-1724234 [Xylella fastidiosa subsp. sandyi Ann-1]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSNDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT45429 NZ_CP006696:c1724539-1724234 [Xylella fastidiosa subsp. sandyi Ann-1]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCCGTCGCTCGTGCTCGTAATTTAA
GTCAGCTGGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Structures

Toxin

• AlphaFold DB: A0A060HBK9