Detailed information of TA system TA045428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1-VagC
• Location: 1720488..1721161
• Replicon: chromosome
• Accession: NZ_CP006696
• Organism: Xylella fastidiosa subsp. sandyi Ann-1

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: D934_RS08045
• Protein ID: WP_024748528.1
• Coordinates: 1720488..1720892 (-)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: D934_RS08050
• Protein ID: WP_024748527.1
• Coordinates: 1720889..1721161 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D934_RS08020	1715544..1716077	+	534	WP_020852388.1	hypothetical protein	-
D934_RS08025	1716078..1717574	+	1497	WP_020852389.1	DUF3383 domain-containing protein	-
D934_RS08030	1717584..1718021	+	438	WP_004087249.1	DUF3277 family protein	-
D934_RS08035	1718018..1718440	+	423	WP_020851660.1	hypothetical protein	-
D934_RS08040	1718588..1720477	+	1890	WP_020851659.1	phage-related protein	-
D934_RS08045	1720488..1720892	-	405	WP_024748528.1	type II toxin-antitoxin system VapC family toxin	Toxin
D934_RS08050	1720889..1721161	-	273	WP_024748527.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
D934_RS08055	1721321..1722091	+	771	WP_020851656.1	hypothetical protein	-
D934_RS08060	1722091..1722408	+	318	WP_020851655.1	hypothetical protein	-
D934_RS08065	1722405..1723235	+	831	WP_020851654.1	hypothetical protein	-
D934_RS08070	1723232..1723873	+	642	WP_020851653.1	phage-related protein	-
D934_RS08075	1723870..1724223	+	354	WP_020851652.1	hypothetical protein	-
D934_RS08080	1724234..1724539	-	306	WP_004089254.1	putative addiction module antidote protein	-
D934_RS08085	1724543..1724839	-	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	-
D934_RS08090	1724938..1726083	+	1146	WP_020851651.1	baseplate J/gp47 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1675562..1727807	52245

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-124)

Antitoxin

• SpoVT-AbrB domain (16-53)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14653.95 Da
• Isoelectric Point: 5.6881

>T45428 WP_024748528.1 NZ_CP006696:c1720892-1720488 [Xylella fastidiosa subsp. sandyi Ann-1]
VSGYMLDTNIISDIIRNPFGAVACRIEHVGYPNICTSVIVAAELRYGCTKNGSVKLLSRVQDILKTLPILPLDIPVDTTY
GSIRAELEAAGQLIGANDLLIAAHAYVLGLTLVTDNTREFSRIRGLDVQNWLER

Nucleotide

• Length: 405 bp

>T45428 NZ_CP006696:c1720892-1720488 [Xylella fastidiosa subsp. sandyi Ann-1]
GTGAGCGGCTATATGCTCGACACAAATATCATCAGCGATATCATTCGTAACCCATTCGGGGCGGTGGCTTGCCGTATTGA
ACACGTTGGGTATCCGAACATTTGCACGAGCGTTATCGTGGCGGCCGAACTTCGCTATGGATGCACGAAAAATGGCTCGG
TAAAGCTGCTGAGCAGGGTACAGGACATTCTTAAAACGCTGCCGATCCTGCCTTTGGACATACCTGTTGACACAACATAT
GGCAGTATCCGTGCTGAGCTGGAGGCTGCGGGTCAGCTGATCGGCGCTAATGATCTATTGATTGCTGCGCATGCTTACGT
ACTCGGGCTGACTTTGGTCACGGACAACACCCGCGAGTTCAGCCGAATTCGCGGCTTGGATGTGCAAAACTGGCTAGAGA
GGTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10396.87 Da
• Isoelectric Point: 4.8782

>AT45428 WP_024748527.1 NZ_CP006696:c1721161-1720889 [Xylella fastidiosa subsp. sandyi Ann-1]
MPISSPLSRTKEAKLFRNNRSQAVRIPVEFELSGDRVLIHREGSKLIIEPITRPTNIVELLHEWRRETPLGPEDQFPDID
DMPAQPEDIL

Nucleotide

• Length: 273 bp

>AT45428 NZ_CP006696:c1721161-1720889 [Xylella fastidiosa subsp. sandyi Ann-1]
ATGCCAATTTCATCTCCGTTATCACGTACTAAGGAAGCGAAGCTATTCCGAAATAATCGTAGCCAGGCGGTACGAATTCC
CGTTGAATTCGAGCTGTCGGGCGATCGTGTTCTGATCCACCGTGAGGGGAGCAAGCTCATTATTGAGCCGATCACCAGGC
CAACCAATATTGTTGAATTACTGCACGAGTGGCGAAGAGAAACACCACTCGGCCCAGAGGATCAATTCCCTGATATTGAT
GACATGCCCGCTCAGCCAGAGGATATTCTGTGA