Detailed information of TA system TA045427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumA-relB/COG3657-DUF971
• Location: 1381401..1382006
• Replicon: chromosome
• Accession: NZ_CP006696
• Organism: Xylella fastidiosa subsp. sandyi Ann-1

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: B2I5N3
• Locus tag: D934_RS06305
• Protein ID: WP_004089252.1
• Coordinates: 1381710..1382006 (-)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: D934_RS06300
• Protein ID: WP_004089254.1
• Coordinates: 1381401..1381706 (-)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
D934_RS06265	1377655..1378059	-	405	WP_024748528.1	type II toxin-antitoxin system VapC family toxin	-
D934_RS06270	1378056..1378328	-	273	WP_024748527.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
D934_RS06275	1378488..1379258	+	771	WP_020851656.1	hypothetical protein	-
D934_RS06280	1379258..1379575	+	318	WP_020851655.1	hypothetical protein	-
D934_RS06285	1379572..1380402	+	831	WP_020851654.1	hypothetical protein	-
D934_RS06290	1380399..1381040	+	642	WP_020851653.1	phage-related protein	-
D934_RS06295	1381037..1381390	+	354	WP_020851652.1	hypothetical protein	-
D934_RS06300	1381401..1381706	-	306	WP_004089254.1	putative addiction module antidote protein	Antitoxin
D934_RS06305	1381710..1382006	-	297	WP_004089252.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
D934_RS06310	1382105..1383250	+	1146	WP_020851651.1	baseplate J/gp47 family protein	-
D934_RS06315	1383247..1383807	+	561	WP_020851650.1	DUF2612 domain-containing protein	-
D934_RS06320	1383811..1385055	+	1245	WP_024748982.1	phage tail protein	-
D934_RS06325	1385159..1385605	-	447	WP_020852919.1	hypothetical protein	-
D934_RS06330	1385864..1386832	-	969	WP_020852918.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1292345..1385055	92710

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-94)

Antitoxin

• Cro/C1-type helix-turn-helix domain (49-95)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 10790.57 Da
• Isoelectric Point: 10.0509

>T45427 WP_004089252.1 NZ_CP006696:c1382006-1381710 [Xylella fastidiosa subsp. sandyi Ann-1]
MVELIKTSTFDAWINSLRDRKAAARIQARLDRLALGNPGDVKPVGAGISEMRIDHGPGYRIYFMKHGAVLILLLCGGDKS
SQVRDIEQAKALAALWKD

Nucleotide

• Length: 297 bp

>T45427 NZ_CP006696:c1382006-1381710 [Xylella fastidiosa subsp. sandyi Ann-1]
ATGGTCGAACTCATCAAAACCAGCACTTTTGATGCTTGGATAAACAGCTTACGTGACCGCAAAGCAGCCGCCAGAATTCA
AGCGCGTCTTGATCGGCTCGCACTTGGCAATCCCGGCGACGTGAAGCCTGTGGGTGCTGGCATATCAGAAATGAGGATCG
ACCATGGGCCCGGATATCGGATCTATTTTATGAAGCATGGTGCAGTACTGATTCTCTTACTTTGCGGTGGCGATAAATCG
TCACAAGTACGAGATATAGAGCAGGCTAAGGCACTGGCTGCACTGTGGAAGGATTAG

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 10983.37 Da
• Isoelectric Point: 5.0941

>AT45427 WP_004089254.1 NZ_CP006696:c1381706-1381401 [Xylella fastidiosa subsp. sandyi Ann-1]
MNNETFSRYDTADYLKTEEDIAAYMEAVMEEGGRDNPAFIARALGAVARARNLSQLARDVGMSRQGLDKALSNDGNPSFS
TILKVAKALGLRMSFTPSSMS

Nucleotide

• Length: 306 bp

>AT45427 NZ_CP006696:c1381706-1381401 [Xylella fastidiosa subsp. sandyi Ann-1]
ATGAATAATGAGACTTTTAGTCGATACGACACCGCTGACTATCTCAAAACCGAAGAAGATATTGCGGCTTATATGGAAGC
CGTCATGGAAGAAGGTGGTAGGGATAATCCTGCTTTTATTGCACGGGCACTTGGTGCCGTCGCTCGTGCTCGTAATTTAA
GTCAGCTGGCTCGTGACGTTGGCATGTCCAGGCAAGGACTAGACAAGGCACTCTCTAACGACGGCAACCCAAGCTTCTCA
ACCATCTTAAAAGTGGCTAAAGCGCTTGGTTTACGGATGTCATTCACACCTTCCTCTATGAGTTAA

Structures

Toxin

• AlphaFold DB: A0A060HBK9