Detailed information of TA system TA045422

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 3452183..3452814
• Replicon: chromosome
• Accession: NZ_CP006694
• Organism: Leptospira santarosai serovar Shermani str. LT 821

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: K8XZE5
• Locus tag: LSS_RS16215
• Protein ID: WP_004460443.1
• Coordinates: 3452413..3452814 (+)
• Length: 134 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: LSS_RS20270
• Protein ID: WP_080600619.1
• Coordinates: 3452183..3452416 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSS_RS16185	3448307..3448954	-	648	WP_004460452.1	transglycosylase domain-containing protein	-
LSS_RS16190	3448957..3449619	-	663	WP_004460451.1	RibD family protein	-
LSS_RS16195	3449616..3449843	-	228	WP_004476676.1	(2Fe-2S)-binding protein	-
LSS_RS16200	3449939..3451339	+	1401	WP_004460447.1	MFS transporter	-
LSS_RS16205	3451385..3451810	+	426	WP_004460445.1	GNAT family N-acetyltransferase	-
LSS_RS16210	3451963..3452172	+	210	WP_002629717.1	hypothetical protein	-
LSS_RS20270	3452183..3452416	+	234	WP_080600619.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LSS_RS16215	3452413..3452814	+	402	WP_004460443.1	type II toxin-antitoxin system VapC family toxin	Toxin
LSS_RS16225	3453339..3454133	-	795	WP_004481521.1	hypothetical protein	-
LSS_RS16230	3454178..3455434	+	1257	WP_004460439.1	hypothetical protein	-
LSS_RS16235	3455528..3456112	+	585	WP_004460438.1	cytochrome C oxidase subunit IV family protein	-
LSS_RS16240	3456299..3456943	+	645	WP_004460436.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-125)

Antitoxin

• SpoVT-AbrB domain (11-49)

Sequences

Toxin

Protein

• Length: 134 a.a.
• Molecular weight: 15539.30 Da
• Isoelectric Point: 9.5697

>T45422 WP_004460443.1 NZ_CP006694:3452413-3452814 [Leptospira santarosai serovar Shermani str. LT 821]
MTHYLLDTNICIYIINQKPESVYKKFKKIKLENIFISSITEFELKYGVQKDLHFERNLKVLEEFLGYLNILQFVSKDASK
AAKIRVELERVGKPIGPFDLLIASQALANKLTLVTNNEKEFARIKEIKMESWL

Nucleotide

• Length: 402 bp

>T45422 NZ_CP006694:3452413-3452814 [Leptospira santarosai serovar Shermani str. LT 821]
ATGACCCACTATTTGTTGGATACGAATATTTGTATTTATATAATAAATCAAAAACCAGAATCTGTTTATAAGAAATTTAA
GAAGATTAAATTAGAGAACATTTTTATTTCATCAATTACAGAATTTGAGCTAAAGTATGGCGTTCAAAAAGATCTTCATT
TTGAGAGGAATTTAAAAGTCTTAGAAGAGTTTCTGGGTTACTTGAATATTCTTCAATTCGTTAGCAAAGACGCTAGTAAA
GCCGCAAAAATTCGAGTAGAGTTAGAAAGAGTAGGTAAACCAATAGGTCCATTTGATCTTTTAATAGCGTCTCAAGCATT
GGCAAATAAGCTTACTCTTGTGACAAACAACGAAAAAGAATTCGCTAGAATTAAAGAGATAAAAATGGAAAGCTGGCTTT
AA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 9194.26 Da
• Isoelectric Point: 7.0092

>AT45422 WP_080600619.1 NZ_CP006694:3452183-3452416 [Leptospira santarosai serovar Shermani str. LT 821]
IPHYSKERLQGDSQAIRLPKNYRFKGREVYIRKDGDNVILTPIDDIVDRFWNTLNNFSDDLIIDRNQPNEYDKRNGI

Nucleotide

• Length: 234 bp

>AT45422 NZ_CP006694:3452183-3452416 [Leptospira santarosai serovar Shermani str. LT 821]
ATTCCTCATTATTCTAAAGAGCGCCTACAAGGCGATAGCCAGGCAATTCGCCTGCCAAAAAACTATCGTTTTAAAGGCAG
AGAAGTCTATATACGAAAAGACGGGGACAATGTTATTTTAACTCCAATAGATGATATCGTGGATCGATTTTGGAATACGC
TTAATAATTTTTCTGACGATCTCATAATAGATCGAAACCAACCAAATGAGTATGACAAAAGAAATGGAATATGA

Structures

Toxin

• AlphaFold DB: K8XZE5