Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 2742616..2743244 Replicon chromosome
Accession NZ_CP006694
Organism Leptospira santarosai serovar Shermani str. LT 821

Toxin (Protein)


Gene name vapC Uniprot ID K8Y570
Locus tag LSS_RS12990 Protein ID WP_004463182.1
Coordinates 2742616..2743014 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID K8XVK2
Locus tag LSS_RS12995 Protein ID WP_004463184.1
Coordinates 2743014..2743244 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LSS_RS12970 2738137..2739027 + 891 WP_004463175.1 hypothetical protein -
LSS_RS12975 2739024..2740421 + 1398 WP_004463176.1 hypothetical protein -
LSS_RS12980 2740711..2741355 + 645 WP_016551945.1 tetratricopeptide repeat protein -
LSS_RS19900 2741644..2741925 + 282 WP_032927939.1 type II toxin-antitoxin system RelE/ParE family toxin -
LSS_RS12985 2741941..2742237 + 297 WP_004463181.1 HigA family addiction module antidote protein -
LSS_RS12990 2742616..2743014 - 399 WP_004463182.1 type II toxin-antitoxin system VapC family toxin Toxin
LSS_RS12995 2743014..2743244 - 231 WP_004463184.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
LSS_RS13000 2743614..2744759 + 1146 WP_049592321.1 IS110 family transposase -
LSS_RS19910 2744883..2745323 - 441 WP_004461853.1 hypothetical protein -
LSS_RS13010 2745368..2748112 - 2745 WP_004461855.1 isoleucine--tRNA ligase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 2743614..2744759 1145


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-123)

Antitoxin

SpoVT-AbrB domain

(7-50)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14980.51 Da        Isoelectric Point: 9.4739

>T45421 WP_004463182.1 NZ_CP006694:c2743014-2742616 [Leptospira santarosai serovar Shermani str. LT 821]
MYLLDTNICIFLIKKKSQKLLDKLKKNFNKGLYISSLTLAELEFGIENSEQKEKNRVSLIEFLSIFEILPFEQSDAQAFG
ILKADLKKSGNPIGSIDALLAAQALSRVLVLITNNTKEFKRVKNLSIEDWSQ

Download         Length: 399 bp

>T45421 NZ_CP006694:c2743014-2742616 [Leptospira santarosai serovar Shermani str. LT 821]
ATGTATCTACTTGATACGAATATTTGTATCTTTCTCATTAAAAAGAAGAGTCAAAAATTATTAGATAAATTAAAGAAGAA
CTTCAACAAAGGTCTGTATATTTCATCGCTAACTTTGGCTGAATTGGAATTTGGCATTGAGAATAGTGAACAAAAAGAAA
AAAATAGGGTATCGTTAATTGAATTTCTTTCCATATTTGAAATTCTTCCGTTTGAGCAATCTGATGCACAAGCATTCGGA
ATTCTTAAGGCTGATTTAAAGAAATCAGGTAATCCTATTGGATCCATTGACGCTCTATTGGCAGCCCAGGCATTGTCGAG
AGTTTTAGTATTGATTACTAATAATACTAAAGAATTTAAGAGAGTAAAGAATCTCAGCATTGAAGATTGGTCTCAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8799.11 Da        Isoelectric Point: 8.7070

>AT45421 WP_004463184.1 NZ_CP006694:c2743244-2743014 [Leptospira santarosai serovar Shermani str. LT 821]
MHTAKLFINGRSQAVRLPKEFQFKGDDVFIQKIGEAVILVPKNKAWNVFLDGLNGFTEDFLKEGREGRLETEREKF

Download         Length: 231 bp

>AT45421 NZ_CP006694:c2743244-2743014 [Leptospira santarosai serovar Shermani str. LT 821]
ATGCACACTGCAAAGTTATTTATTAACGGAAGAAGTCAAGCTGTAAGACTTCCAAAAGAATTTCAGTTTAAAGGTGACGA
TGTTTTTATTCAAAAAATAGGTGAAGCTGTAATTTTAGTTCCTAAGAATAAAGCGTGGAACGTTTTCCTCGATGGACTTA
ACGGATTTACTGAAGATTTTCTAAAAGAAGGAAGAGAAGGGCGCCTCGAAACTGAAAGAGAAAAATTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2R4L8Y5

Antitoxin

Source ID Structure
AlphaFold DB K8XVK2

References