Detailed information of TA system TA045420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpIK/PemK(toxin)
• Location: 2567319..2567895
• Replicon: chromosome
• Accession: NZ_CP006694
• Organism: Leptospira santarosai serovar Shermani str. LT 821

Toxin (Protein)

• Gene name: chpK
• Uniprot ID: K8XVX0
• Locus tag: LSS_RS12100
• Protein ID: WP_004462034.1
• Coordinates: 2567319..2567660 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: chpI
• Uniprot ID: M6JN71
• Locus tag: LSS_RS12105
• Protein ID: WP_004462036.1
• Coordinates: 2567647..2567895 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSS_RS12085	2562830..2563219	-	390	WP_002762646.1	response regulator	-
LSS_RS12090	2563342..2565051	-	1710	WP_004462031.1	EAL domain-containing protein	-
LSS_RS12095	2565244..2566854	+	1611	WP_004462033.1	2Fe-2S iron-sulfur cluster binding domain-containing protein	-
LSS_RS12100	2567319..2567660	-	342	WP_004462034.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LSS_RS12105	2567647..2567895	-	249	WP_004462036.1	ribbon-helix-helix domain-containing protein	Antitoxin
LSS_RS12110	2568328..2568660	-	333	WP_004462038.1	helix-turn-helix domain-containing protein	-
LSS_RS12115	2568644..2568814	-	171	WP_004462040.1	hypothetical protein	-
LSS_RS21380	2568916..2569089	-	174	WP_016552099.1	hypothetical protein	-
LSS_RS12120	2569306..2569794	-	489	WP_004462044.1	hypothetical protein	-
LSS_RS12125	2569973..2570197	-	225	WP_004462046.1	hypothetical protein	-
LSS_RS12130	2570194..2570376	-	183	WP_032920397.1	hypothetical protein	-
LSS_RS12135	2570579..2571682	+	1104	WP_004462049.1	histidinol-phosphate transaminase	-
LSS_RS21385	2571949..2572089	+	141	WP_004462050.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-112)

Antitoxin

• Predicted DNA-binding protein, ribbon-helix-helix domain (2-41)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12424.24 Da
• Isoelectric Point: 8.6584

>T45420 WP_004462034.1 NZ_CP006694:c2567660-2567319 [Leptospira santarosai serovar Shermani str. LT 821]
MTRGEIWWVDLGIPFGSEPGFKRPVLIIQDDSFNRSNINTIVSIAVTSNLNLSEAPGNVLITKKDSNLSKDSVVNVSQIV
TLDKERFLNKAGKLKTNKLNEVGEGLRLVTGLE

Nucleotide

• Length: 342 bp

>T45420 NZ_CP006694:c2567660-2567319 [Leptospira santarosai serovar Shermani str. LT 821]
ATGACTCGTGGTGAAATTTGGTGGGTAGATTTGGGAATTCCTTTTGGAAGTGAACCTGGATTTAAACGTCCAGTTCTTAT
AATTCAAGACGATTCCTTTAATCGAAGTAATATTAATACAATTGTGTCGATTGCCGTCACATCAAATTTGAATTTATCTG
AAGCGCCAGGAAATGTTTTAATTACTAAAAAAGATTCAAATTTATCGAAGGATTCAGTGGTAAATGTTTCCCAAATCGTG
ACTTTAGATAAAGAAAGATTTCTGAACAAAGCAGGAAAACTGAAAACGAATAAATTGAATGAAGTCGGAGAGGGGCTCAG
GTTGGTTACGGGCTTAGAGTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9385.79 Da
• Isoelectric Point: 10.3009

>AT45420 WP_004462036.1 NZ_CP006694:c2567895-2567647 [Leptospira santarosai serovar Shermani str. LT 821]
MKTAISIPDELFKTAEKTAKKLGIPRSQLFAKALEEFIRSHSKESVTEKLNKIYDNKSKETKTNLTDLSVESLRKSLKND
SW

Nucleotide

• Length: 249 bp

>AT45420 NZ_CP006694:c2567895-2567647 [Leptospira santarosai serovar Shermani str. LT 821]
ATGAAAACTGCAATTTCGATTCCCGATGAGCTATTTAAAACTGCAGAGAAAACGGCTAAAAAACTTGGTATTCCTAGAAG
CCAGTTATTTGCAAAAGCATTAGAAGAATTTATTCGGTCTCATAGTAAGGAATCCGTAACTGAGAAATTAAATAAAATCT
ACGATAATAAATCTAAAGAAACCAAAACGAATCTTACCGATTTATCCGTAGAATCACTTCGCAAGAGTTTAAAAAATGAC
TCGTGGTGA

Structures

Toxin

• AlphaFold DB: K8XVX0

Antitoxin

• AlphaFold DB: M6JN71