Detailed information of TA system TA045418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 1081661..1082226
• Replicon: chromosome
• Accession: NZ_CP006694
• Organism: Leptospira santarosai serovar Shermani str. LT 821

Toxin (Protein)

• Gene name: mazE
• Uniprot ID: K8YH10
• Locus tag: LSS_RS05160
• Protein ID: WP_004458030.1
• Coordinates: 1081661..1081984 (-)
• Length: 108 a.a.

Antitoxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: LSS_RS05165
• Protein ID: WP_026053376.1
• Coordinates: 1081984..1082226 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LSS_RS05145	1077553..1078248	-	696	WP_004458038.1	ABC transporter ATP-binding protein	-
LSS_RS05150	1078606..1079730	-	1125	WP_004458035.1	SpoIID/LytB domain-containing protein	-
LSS_RS05155	1079902..1081257	-	1356	WP_004458032.1	class I SAM-dependent RNA methyltransferase	-
LSS_RS05160	1081661..1081984	-	324	WP_004458030.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
LSS_RS05165	1081984..1082226	-	243	WP_026053376.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
LSS_RS05170	1082658..1083767	-	1110	WP_004458026.1	DUF1565 domain-containing protein	-
LSS_RS05175	1084378..1085358	-	981	WP_016551843.1	transporter	-
LSS_RS05180	1085358..1086236	-	879	WP_004458024.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (2-105)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 108 a.a.
• Molecular weight: 12250.67 Da
• Isoelectric Point: 10.3519

>T45418 WP_004458030.1 NZ_CP006694:c1081984-1081661 [Leptospira santarosai serovar Shermani str. LT 821]
MVIAQYEVYLINLDPTIGYEIKKSRPCVIISPNEMNKTIGTIIIAPMTTKSRTYPTRIELTFQGKKGWIVLDQIRTVDKV
RLIKKLGKIDSKISNQLKSIIKEMLVD

Nucleotide

• Length: 324 bp

>T45418 NZ_CP006694:c1081984-1081661 [Leptospira santarosai serovar Shermani str. LT 821]
ATGGTGATTGCCCAATACGAAGTATATTTGATAAATTTAGATCCAACGATCGGATATGAGATTAAAAAATCAAGGCCCTG
TGTAATAATTTCGCCTAATGAAATGAATAAAACGATTGGAACGATTATTATCGCACCAATGACGACCAAATCTAGAACTT
ATCCAACTAGGATTGAATTAACATTTCAAGGAAAGAAAGGTTGGATTGTGTTAGATCAAATTCGAACCGTAGATAAGGTT
CGCTTGATAAAAAAGTTGGGTAAAATAGATTCCAAAATCAGTAATCAATTGAAATCGATTATAAAAGAAATGTTGGTTGA
CTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9202.70 Da
• Isoelectric Point: 5.8481

>AT45418 WP_026053376.1 NZ_CP006694:c1082226-1081984 [Leptospira santarosai serovar Shermani str. LT 821]
MKVSVVKIGNSKGIRIPKAVLEECHIEDEVDLLINNNKLIVVPLKSKPREGWEKQFKTMSSNKDDKLLIPDSIDLSDWEW

Nucleotide

• Length: 243 bp

>AT45418 NZ_CP006694:c1082226-1081984 [Leptospira santarosai serovar Shermani str. LT 821]
ATGAAGGTCTCGGTTGTAAAAATAGGTAATTCTAAAGGTATTCGAATACCCAAGGCGGTTTTAGAAGAATGTCACATTGA
AGACGAAGTAGATTTGCTAATCAATAATAACAAACTAATCGTCGTACCGCTAAAATCAAAACCAAGAGAAGGATGGGAAA
AACAATTTAAGACAATGTCGTCTAATAAAGATGATAAATTACTTATTCCTGATTCAATAGATTTGTCAGATTGGGAATGG
TGA

Structures

Toxin

• AlphaFold DB: A0A2R4L4R9