Detailed information of TA system TA045392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 48364..49100
• Replicon: plasmid pCuAs
• Accession: NZ_CP006663
• Organism: Klebsiella pneumoniae strain ATCC BAA-2146

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: L7SZ15
• Locus tag: kpn2146_RS29090
• Protein ID: WP_003026803.1
• Coordinates: 48364..48846 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: kpn2146_RS29095
• Protein ID: WP_003026799.1
• Coordinates: 48834..49100 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
kpn2146_RS29075	43718..45481	-	1764	WP_004200842.1	DUF262 domain-containing protein	-
kpn2146_RS30885	45679..45885	-	207	Protein_45	helix-turn-helix domain-containing protein	-
kpn2146_RS29080	46099..46731	+	633	WP_001567369.1	hypothetical protein	-
kpn2146_RS29085	46760..48163	-	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
kpn2146_RS29090	48364..48846	-	483	WP_003026803.1	GNAT family N-acetyltransferase	Toxin
kpn2146_RS29095	48834..49100	-	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
kpn2146_RS30890	49345..49779	-	435	WP_001567367.1	cell envelope integrity protein TolA	-
kpn2146_RS30895	49826..50374	-	549	WP_001567366.1	thioredoxin fold domain-containing protein	-
kpn2146_RS29105	50868..51176	+	309	WP_017896554.1	hypothetical protein	-
kpn2146_RS29110	52075..53613	-	1539	WP_004201219.1	IS66-like element ISKpn24 family transposase	-
kpn2146_RS29115	53662..54009	-	348	WP_000612626.1	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	mph(A) / tet(A)	-	1..117755	117755
	-	inside	IScluster/Tn	-	-	46760..54410	7650

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-149)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17353.97 Da
• Isoelectric Point: 9.5822

>T45392 WP_003026803.1 NZ_CP006663:c48846-48364 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Nucleotide

• Length: 483 bp

>T45392 NZ_CP006663:c48846-48364 [Klebsiella pneumoniae]
GTGGGACGTATAACCACGCCCGAGCCGTTATCCAGCTCTCATCAGCTGGCTGAGTTCGTCAGTGGAGAGACAGTCCTCGA
TGAATGGCTAAAACAGAGAGGTTTAAAAAATCAAGCTCTTGGCGCTGCCCGAACGTTCGTTGTTTGCAAGACGGGTACGA
AGCAAGTAGCTGGTTTTTACTCTTTGGCCACCGGTAGCGTTAACCATACGGAAGCGACAGGTAGTCTTCGGCGTAACATG
CCAGATCCTATACCGGTGATTATACTCGCTCGCCTGGCGGTAGATGTCTCATTGCACGGAAAAGGGGTTGGCGCCGATTT
ACTCCACGATGCAGTTCTACGGTGTTATCGCGTAGCTGAGAACATTGGGGTTCGTGCGATAATGGTTCATGCGCTTACTG
AAGAAGCCAAAGGTTTCTATGCTCACCATGGATTTAAGGCATCACAAACTCATGAGAGGACTCTGTTTCTAAAGCTTCCA
TGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT45392 WP_003026799.1 NZ_CP006663:c49100-48834 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT45392 NZ_CP006663:c49100-48834 [Klebsiella pneumoniae]
ATGAAATCAGATGTTCAACTCAACCTAAGAGCTAAAGAGTCTCAGAGAGCACTCATCGATGCTGCTGCAGAGATCCTTCA
TAAATCGCGAACCGACTTTATTCTGGAGATGGCCTGCAAGGCTGCGGAGAATGTGATCCTTGATCGCCGTGTTTTCAATT
TTAACGACGAACAGTATGCAGAGTTCATCGATATGCTCGATGCACCAGTCGAGGATGAGCCCGCCATTAATAAACTACTG
GCAAGGAAACCTCAGTGGGACGTATAA

Structures

Toxin

• AlphaFold DB: A0A0J2GNW6

Antitoxin

• AlphaFold DB: A0A1Q8YL66