Detailed information of TA system TA045391

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 80306..80949
• Replicon: plasmid pHg
• Accession: NZ_CP006662
• Organism: Klebsiella pneumoniae strain ATCC BAA-2146

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: D8L2J9
• Locus tag: kpn2146_RS28805
• Protein ID: WP_001044770.1
• Coordinates: 80533..80949 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: D5KTK7
• Locus tag: kpn2146_RS28800
• Protein ID: WP_001261282.1
• Coordinates: 80306..80536 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
kpn2146_RS33405	76070..76174	+	105	WP_032409716.1	hypothetical protein	-
kpn2146_RS28765	76303..76560	+	258	WP_000764642.1	hypothetical protein	-
kpn2146_RS28770	76618..77394	-	777	WP_000015958.1	site-specific integrase	-
kpn2146_RS28775	77391..78134	-	744	WP_000129823.1	hypothetical protein	-
kpn2146_RS28780	78185..78535	-	351	WP_000493378.1	hypothetical protein	-
kpn2146_RS28785	79343..79795	+	453	WP_004201034.1	hypothetical protein	-
kpn2146_RS33410	80122..80349	-	228	Protein_93	hypothetical protein	-
kpn2146_RS28800	80306..80536	+	231	WP_001261282.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
kpn2146_RS28805	80533..80949	+	417	WP_001044770.1	type II toxin-antitoxin system VapC family toxin	Toxin
kpn2146_RS28810	81023..81733	+	711	WP_004152334.1	ATP-binding protein	-
kpn2146_RS28815	81767..82464	+	698	Protein_97	IS1 family transposase	-
kpn2146_RS30780	82481..82672	-	192	Protein_98	nucleotidyltransferase domain-containing protein	-
kpn2146_RS28820	82742..83296	-	555	WP_063840280.1	AAC(6')-Ib family aminoglycoside 6'-N-acetyltransferase	-
kpn2146_RS28825	83530..84087	-	558	WP_001217881.1	recombinase family protein	-
kpn2146_RS28830	84393..85097	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	dfrA14 / qnrB9 / blaSHV-187 / aac(6')-Ib-cr / blaOXA-1 / aac(3)-IIa / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / aac(6')-Ib	-	1..85161	85161

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15106.52 Da
• Isoelectric Point: 7.1084

>T45391 WP_001044770.1 NZ_CP006662:80533-80949 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR

Nucleotide

• Length: 417 bp

>T45391 NZ_CP006662:80533-80949 [Klebsiella pneumoniae]
GTGAACAAGACCTATATGCTCGACACCTGTATCTGCTCGTTCATCATGCGCGAGCAGCCGGAAGCCGTCCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCAGCGCATCGTTGTCTCGGCCATCACTTATTCTGAAATGCGCTTCGGGGCCACCGGCC
CGAAGGCCTCCCCGCGCCACGTCGAGCTGGTCGACGCGTTCTGCGCCCGCCTCGATGCCATCCTGCCCTGGGATCGCGCT
GCGGTGGACGCCACCACGGAGATTAAGGTGGCGCTGCGAATGGCCGGGACGCCGATCGGCCCGAACGACACGGCCATTGC
CGGGCACGCCATCGCCGCCGGTGCCGTGCTGGTGACGAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8756.83 Da
• Isoelectric Point: 4.4692

>AT45391 WP_001261282.1 NZ_CP006662:80306-80536 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL

Nucleotide

• Length: 231 bp

>AT45391 NZ_CP006662:80306-80536 [Klebsiella pneumoniae]
ATGAGAACTGTATCCATTTTTAAAAACGGCAATAACCGCGCCATTCGTCTGCCCCGCGATCTGGATTTTGAGGGAGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATTATTTTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGTTCG
AGAAGGCAGACCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0H3GYM2

Antitoxin

• AlphaFold DB: A0A0H3GZG3