Detailed information of TA system TA045389

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 5073140..5073726
• Replicon: chromosome
• Accession: NZ_CP006659
• Organism: Klebsiella pneumoniae strain ATCC BAA-2146

Toxin (Protein)

• Gene name: doc
• Uniprot ID: W8VD46
• Locus tag: kpn2146_RS25715
• Protein ID: WP_002920800.1
• Coordinates: 5073140..5073508 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: A0A0H3GZM4
• Locus tag: kpn2146_RS25720
• Protein ID: WP_002920802.1
• Coordinates: 5073505..5073726 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
kpn2146_RS25695	5068643..5069713	-	1071	WP_002920787.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-
kpn2146_RS25700	5069715..5070560	-	846	WP_002920789.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
kpn2146_RS25705	5070557..5071444	-	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
kpn2146_RS25710	5071551..5072867	-	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
kpn2146_RS25715	5073140..5073508	-	369	WP_002920800.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
kpn2146_RS25720	5073505..5073726	-	222	WP_002920802.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
kpn2146_RS25725	5073890..5074603	-	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
kpn2146_RS25730	5074605..5075372	-	768	WP_004200668.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
kpn2146_RS25735	5075369..5076646	-	1278	WP_002920806.1	branched chain amino acid ABC transporter permease LivM	-
kpn2146_RS25740	5076643..5077569	-	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	5067906..5104981	37075

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-49)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T45389 WP_002920800.1 NZ_CP006659:c5073508-5073140 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T45389 NZ_CP006659:c5073508-5073140 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCAGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACTCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCGGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGCTTGCGCGGATGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8181.18 Da
• Isoelectric Point: 4.7769

>AT45389 WP_002920802.1 NZ_CP006659:c5073726-5073505 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQTARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT45389 NZ_CP006659:c5073726-5073505 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGACGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A0H3GUD1

Antitoxin

• AlphaFold DB: A0A0H3GZM4