45376

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 1248575..1249194 Replicon chromosome
Accession NZ_CP006659
Organism Klebsiella pneumoniae strain ATCC BAA-2146

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag kpn2146_RS06210 Protein ID WP_002892050.1
Coordinates 1248575..1248793 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag kpn2146_RS06215 Protein ID WP_002892066.1
Coordinates 1248820..1249194 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
kpn2146_RS06175 1244622..1244885 + 264 WP_002892011.1 type B 50S ribosomal protein L31 -
kpn2146_RS06180 1244885..1245025 + 141 WP_002892018.1 type B 50S ribosomal protein L36 -
kpn2146_RS06185 1245022..1245720 - 699 WP_002892021.1 GNAT family N-acetyltransferase -
kpn2146_RS06190 1245821..1247272 + 1452 WP_002892023.1 PLP-dependent aminotransferase family protein -
kpn2146_RS06195 1247247..1247717 - 471 WP_002892026.1 YlaC family protein -
kpn2146_RS32315 1247738..1247878 + 141 WP_004147370.1 hypothetical protein -
kpn2146_RS06205 1247850..1248416 - 567 WP_002892030.1 maltose O-acetyltransferase -
kpn2146_RS06210 1248575..1248793 - 219 WP_002892050.1 hemolysin expression modulator Hha Toxin
kpn2146_RS06215 1248820..1249194 - 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
kpn2146_RS06220 1249680..1252826 - 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
kpn2146_RS06225 1252849..1254042 - 1194 WP_002892072.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T45376 WP_002892050.1 NZ_CP006659:c1248793-1248575 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T45376 NZ_CP006659:c1248793-1248575 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACTTCTGTCTGGAAATTTATCCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT45376 WP_002892066.1 NZ_CP006659:c1249194-1248820 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT45376 NZ_CP006659:c1249194-1248820 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References