Detailed information of TA system TA045363

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 517096..517720
• Replicon: chromosome
• Accession: NZ_CP006644
• Organism: Sphingomonas sanxanigenens DSM 19645 = NX02

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: NX02_RS02335
• Protein ID: WP_025290600.1
• Coordinates: 517412..517720 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: NX02_RS02330
• Protein ID: WP_197421464.1
• Coordinates: 517096..517380 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
NX02_RS02310	512412..513035	+	624	WP_025290597.1	TetR/AcrR family transcriptional regulator	-
NX02_RS30495	513112..514236	-	1125	WP_211258271.1	helix-turn-helix domain-containing protein	-
NX02_RS02320	514374..515528	-	1155	WP_158013865.1	hypothetical protein	-
NX02_RS02325	515609..517048	+	1440	WP_211258272.1	aldehyde dehydrogenase family protein	-
NX02_RS02330	517096..517380	-	285	WP_197421464.1	BrnA antitoxin family protein	Antitoxin
NX02_RS02335	517412..517720	-	309	WP_025290600.1	BrnT family toxin	Toxin
NX02_RS02340	517748..518335	-	588	WP_025290601.1	recombinase family protein	-
NX02_RS02345	518765..519166	-	402	WP_004207001.1	DUF2147 domain-containing protein	-
NX02_RS02350	519179..520771	-	1593	WP_025290602.1	GMC family oxidoreductase N-terminal domain-containing protein	-
NX02_RS31195	520776..521309	-	534	WP_082733396.1	SMI1/KNR4 family protein	-
NX02_RS31200	521421..522620	-	1200	Protein_485	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	489078..593397	104319
	-	flank	IS/Tn	-	-	517748..518335	587

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (8-93)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (1-82)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11904.39 Da
• Isoelectric Point: 6.9916

>T45363 WP_025290600.1 NZ_CP006644:c517720-517412 [Sphingomonas sanxanigenens DSM 19645 = NX02]
MPSMRFSWHPAKAESNLKKHGVSFAIATRAFTDRFALSDQDRIEGGERRWQTLGLVEGHLLLLVAHTVVEQDDDGEYVEI
IHIISARKADRTERRRYEEERR

Nucleotide

• Length: 309 bp

>T45363 NZ_CP006644:c517720-517412 [Sphingomonas sanxanigenens DSM 19645 = NX02]
ATGCCATCCATGCGCTTCTCCTGGCATCCGGCCAAAGCCGAGAGCAATCTGAAGAAGCACGGCGTCAGCTTTGCGATCGC
TACTCGTGCGTTCACAGATCGTTTCGCGCTGAGCGATCAGGATCGGATCGAAGGTGGCGAGCGACGATGGCAAACCTTGG
GCCTCGTGGAAGGGCATCTGCTCCTCCTCGTGGCCCACACTGTCGTCGAGCAAGACGATGACGGCGAGTATGTGGAGATC
ATTCATATCATTTCGGCTCGAAAGGCCGATCGAACGGAAAGGCGACGTTATGAAGAAGAAAGACGCTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10764.24 Da
• Isoelectric Point: 9.9870

>AT45363 WP_197421464.1 NZ_CP006644:c517380-517096 [Sphingomonas sanxanigenens DSM 19645 = NX02]
MPPLTEAQQAELDALQAMPDSGIDYSDSPALTEDFWRNGQRGRFYKPIKQQVTARLDADVLAWLKSQGKGYQARMNAILR
REMLATARRQEKPR

Nucleotide

• Length: 285 bp

>AT45363 NZ_CP006644:c517380-517096 [Sphingomonas sanxanigenens DSM 19645 = NX02]
CTGCCGCCGTTGACCGAAGCGCAACAGGCCGAACTGGATGCCCTGCAGGCGATGCCGGATAGCGGGATCGACTATAGCGA
CTCTCCGGCGCTGACAGAGGATTTCTGGCGGAACGGGCAGCGTGGGCGCTTCTATAAGCCCATCAAGCAGCAGGTGACGG
CACGCCTTGATGCTGATGTGCTCGCCTGGCTCAAATCGCAGGGTAAGGGCTACCAAGCCCGCATGAACGCCATACTTCGC
CGTGAGATGCTGGCGACCGCCAGAAGGCAGGAAAAGCCACGTTAA