45362

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vagCD/VapC-VagC
Location 90726..91369 Replicon plasmid PCN061p6
Accession NZ_CP006642
Organism Escherichia coli PCN061

Toxin (Protein)


Gene name vagD Uniprot ID Q84A06
Locus tag PCN061_RS24605 Protein ID WP_000754566.1
Coordinates 90953..91369 (+) Length 139 a.a.

Antitoxin (Protein)


Gene name vagC Uniprot ID Q84A07
Locus tag PCN061_RS24600 Protein ID WP_001261276.1
Coordinates 90726..90956 (+) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PCN061_RS24575 86680..87377 + 698 Protein_94 IS1 family transposase -
PCN061_RS24580 87420..88214 - 795 WP_047929052.1 site-specific integrase -
PCN061_RS24585 88449..89431 - 983 Protein_96 hypothetical protein -
PCN061_RS24590 89601..89906 - 306 WP_032437525.1 type II toxin-antitoxin system toxin CcdB -
PCN061_RS24595 89908..90126 - 219 WP_032437523.1 type II toxin-antitoxin system antitoxin CcdA -
PCN061_RS24600 90726..90956 + 231 WP_001261276.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein Antitoxin
PCN061_RS24605 90953..91369 + 417 WP_000754566.1 type II toxin-antitoxin system VapC family toxin Toxin
PCN061_RS28220 91573..92429 + 857 Protein_101 IS3-like element ISEc15 family transposase -
PCN061_RS24620 92711..93787 + 1077 WP_000227969.1 IS110 family transposase -
PCN061_RS24625 93804..94082 + 279 Protein_103 IS3 family transposase -
PCN061_RS24630 94190..96265 - 2076 WP_047929053.1 protease Lon-related BREX system protein BrxL -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / oqxB / oqxA / aac(3)-IId / blaTEM-1B mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..145722 145722
- inside IScluster/Tn - - 83587..94082 10495


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(6-129)

Antitoxin

SpoVT-AbrB domain

(7-47)


Sequences

Toxin        


Download         Length: 139 a.a.        Molecular weight: 15006.52 Da        Isoelectric Point: 9.2957

>T45362 WP_000754566.1 NZ_CP006642:90953-91369 [Escherichia coli PCN061]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAVLPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAILVTNNVREFARVPGLVLEDWVK

Download         Length: 417 bp

>T45362 NZ_CP006642:90953-91369 [Escherichia coli PCN061]
ATGAAGAAAACCTGGATGCTCGACACCAACATCTGCTCGTTCATCATGCGCGAGCAGCCGGCAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGTGATCGCATTGTGGTCTCGGCCGTGACGTATGCCGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGCCATATTCAGCTGGTCGACGCGTTCTGCGCGCGCCTCGATGCCGTCCTGCCCTGGGACCGCGCC
GCGGTGGACGCCACGACGGACATCCGGGTGGCGCTGCGCCTAGCCGGGACGCCGATCGGCCCGAATGACACGGCCATTGC
CGGGCACGCCATCGCGGCGGGCGCCATCCTGGTGACGAACAATGTGAGAGAGTTTGCGCGGGTCCCGGGCCTGGTGCTGG
AAGACTGGGTGAAATAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8721.74 Da        Isoelectric Point: 4.3533

>AT45362 WP_001261276.1 NZ_CP006642:90726-90956 [Escherichia coli PCN061]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Download         Length: 231 bp

>AT45362 NZ_CP006642:90726-90956 [Escherichia coli PCN061]
ATGCGCACAGTTTCAATATTTAAAAACGGCAATAACCGCGCCATCCGTCTGCCCCGGGACCTGGATTTTGATGGCGTCAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGTCCCGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTGG
ACAGGGCCGATCCGGACTTTATGGCGGAGCGTGAGGATGTGGTCAGCGATGAAGGACGCTTTGAGCCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A387K1G3

Antitoxin

Source ID Structure
AlphaFold DB A0A387K023

References