Detailed information of TA system TA045361

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 89601..90126
• Replicon: plasmid PCN061p6
• Accession: NZ_CP006642
• Organism: Escherichia coli PCN061

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A8H9ZYZ5
• Locus tag: PCN061_RS24590
• Protein ID: WP_032437525.1
• Coordinates: 89601..89906 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A853HFQ6
• Locus tag: PCN061_RS24595
• Protein ID: WP_032437523.1
• Coordinates: 89908..90126 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCN061_RS27305	85104..85463	+	360	WP_170877903.1	hypothetical protein	-
PCN061_RS24565	85511..86488	-	978	WP_000600827.1	tyrosine-type recombinase/integrase	-
PCN061_RS24575	86680..87377	+	698	Protein_94	IS1 family transposase	-
PCN061_RS24580	87420..88214	-	795	WP_047929052.1	site-specific integrase	-
PCN061_RS24585	88449..89431	-	983	Protein_96	hypothetical protein	-
PCN061_RS24590	89601..89906	-	306	WP_032437525.1	type II toxin-antitoxin system toxin CcdB	Toxin
PCN061_RS24595	89908..90126	-	219	WP_032437523.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
PCN061_RS24600	90726..90956	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
PCN061_RS24605	90953..91369	+	417	WP_000754566.1	type II toxin-antitoxin system VapC family toxin	-
PCN061_RS28220	91573..92429	+	857	Protein_101	IS3-like element ISEc15 family transposase	-
PCN061_RS24620	92711..93787	+	1077	WP_000227969.1	IS110 family transposase	-
PCN061_RS24625	93804..94082	+	279	Protein_103	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-Ia / oqxB / oqxA / aac(3)-IId / blaTEM-1B	mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA	1..145722	145722
	-	inside	IScluster/Tn	-	-	83587..94082	10495

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11587.24 Da
• Isoelectric Point: 6.4650

>T45361 WP_032437525.1 NZ_CP006642:c89906-89601 [Escherichia coli PCN061]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLSSARLLSDKVSRDLYPVVHIGDDSYRLMTTDMASVTASVTGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T45361 NZ_CP006642:c89906-89601 [Escherichia coli PCN061]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGGCGCATGGTGATCCCGCTGTCCAGCGCCCGGCTGCTGTCGGATAAGGTCTCCCGTGATCTCTATCCGGTGG
TGCACATCGGGGACGACAGCTATCGCCTGATGACCACGGACATGGCCAGCGTCACGGCCTCTGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8420.39 Da
• Isoelectric Point: 4.7558

>AT45361 WP_032437523.1 NZ_CP006642:c90126-89908 [Escherichia coli PCN061]
MKQRITVTIDSDSYQLLKSADVNISRLVNTAMQNEARRLRAERWQAENQEGMAEVARFIEMNGSFAEENRDW

Nucleotide

• Length: 219 bp

>AT45361 NZ_CP006642:c90126-89908 [Escherichia coli PCN061]
ATGAAGCAGCGCATCACCGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCGGACGTCAATATATCCCGTCT
GGTGAACACGGCCATGCAGAATGAGGCCCGCCGTTTGCGTGCAGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGGTTTATTGAAATGAACGGTTCATTCGCCGAAGAAAACAGGGACTGGTGA

Structures

Antitoxin

• AlphaFold DB: A0A853HFQ6