Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 67575..68097 Replicon plasmid PCN061p6
Accession NZ_CP006642
Organism Escherichia coli PCN061

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag PCN061_RS24460 Protein ID WP_000220560.1
Coordinates 67575..67856 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag PCN061_RS24465 Protein ID WP_000121743.1
Coordinates 67846..68097 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PCN061_RS24425 63960..64661 - 702 WP_047929050.1 type 3 fimbria chaperone MrkB -
PCN061_RS24430 64757..65365 - 609 WP_047929051.1 type 3 fimbria major subunit MrkA -
PCN061_RS24440 65647..66344 + 698 Protein_69 IS1 family transposase -
PCN061_RS24445 66611..66826 - 216 WP_001180116.1 hypothetical protein -
PCN061_RS24450 66816..67061 - 246 WP_000356546.1 hypothetical protein -
PCN061_RS24455 67106..67429 - 324 WP_001181903.1 hypothetical protein -
PCN061_RS24460 67575..67856 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
PCN061_RS24465 67846..68097 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
PCN061_RS24470 69254..70090 + 837 WP_001050931.1 replication initiation protein -
PCN061_RS24475 70130..70576 + 447 WP_001074386.1 hypothetical protein -
PCN061_RS24480 70644..71066 + 423 WP_031942371.1 DNA distortion polypeptide 3 -
PCN061_RS24485 71126..72094 - 969 WP_077249520.1 IS5-like element IS903B family transposase -
PCN061_RS24490 72096..72389 - 294 WP_015060510.1 hypothetical protein -
PCN061_RS24495 72405..72635 - 231 WP_000051064.1 partitioning protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / oqxB / oqxA / aac(3)-IId / blaTEM-1B mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..145722 145722
- inside IScluster/Tn - - 65841..78417 12576


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T45360 WP_000220560.1 NZ_CP006642:c67856-67575 [Escherichia coli PCN061]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T45360 NZ_CP006642:c67856-67575 [Escherichia coli PCN061]
ATGACCTATGAACTGGCTTTTGACCGTAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAACTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATACCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCCGTTGGAAGA
AGGGAAAGCAGCGAAGTCTATCAGATCGCAGATTTGCGCTAA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT45360 WP_000121743.1 NZ_CP006642:c68097-67846 [Escherichia coli PCN061]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT45360 NZ_CP006642:c68097-67846 [Escherichia coli PCN061]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G3CAN5

Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References