Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-RelB
Location 42961..43487 Replicon plasmid PCN061p6
Accession NZ_CP006642
Organism Escherichia coli PCN061

Toxin (Protein)


Gene name relE Uniprot ID S1EXL1
Locus tag PCN061_RS24295 Protein ID WP_000323025.1
Coordinates 42961..43248 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q5J3S4
Locus tag PCN061_RS24300 Protein ID WP_000534857.1
Coordinates 43248..43487 (-) Length 80 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PCN061_RS24275 38065..39240 - 1176 WP_002914189.1 multidrug efflux RND transporter periplasmic adaptor subunit OqxA -
PCN061_RS24280 39560..40264 + 705 WP_001067858.1 IS6-like element IS26 family transposase -
PCN061_RS27245 40493..40717 - 225 Protein_39 hypothetical protein -
PCN061_RS24285 40856..41878 + 1023 WP_000627495.1 IS21-like element ISSen3 family transposase -
PCN061_RS24290 41875..42657 + 783 WP_001531258.1 IS21-like element ISSen3 family helper ATPase IstB -
PCN061_RS24295 42961..43248 - 288 WP_000323025.1 type II toxin-antitoxin system mRNA interferase RelE Toxin
PCN061_RS24300 43248..43487 - 240 WP_000534857.1 type II toxin-antitoxin system antitoxin RelB Antitoxin
PCN061_RS29530 43829..43978 + 150 WP_000550473.1 hypothetical protein -
PCN061_RS24310 44142..47108 - 2967 WP_001138064.1 Tn3-like element TnAs3 family transposase -
PCN061_RS24315 47111..47671 - 561 WP_000147567.1 recombinase family protein -
PCN061_RS29135 47797..48147 - 351 WP_000454193.1 DUF3330 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(3')-Ia / oqxB / oqxA / aac(3)-IId / blaTEM-1B mrkJ / mrkF / mrkD / mrkC / mrkB / mrkA 1..145722 145722
- inside IScluster/Tn oqxB / oqxA / aac(3)-IId / blaTEM-1B - 27180..59561 32381


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-86)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(1-79)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11225.22 Da        Isoelectric Point: 10.1967

>T45359 WP_000323025.1 NZ_CP006642:c43248-42961 [Escherichia coli PCN061]
MAYFLDFDERALKEWRKLGSTVREQLKKKLVEVLESPRIEANKLRGMPDCYKIKLRSSGYRLVYQVIDEKVVVFVISVGK
RERSEVYSEAVKRIL

Download         Length: 288 bp

>T45359 NZ_CP006642:c43248-42961 [Escherichia coli PCN061]
ATGGCGTATTTTCTGGATTTTGACGAGCGGGCACTAAAGGAATGGCGAAAGCTGGGCTCGACGGTACGTGAACAGTTGAA
AAAGAAGCTGGTTGAAGTACTTGAGTCACCCCGGATTGAAGCAAACAAGCTCCGTGGTATGCCTGATTGTTACAAGATTA
AGCTCCGGTCTTCAGGCTATCGCCTTGTATACCAGGTTATAGACGAGAAAGTTGTCGTTTTCGTGATTTCTGTTGGGAAA
AGAGAACGCTCGGAAGTATATAGCGAGGCGGTCAAACGCATTCTCTGA

Antitoxin


Download         Length: 80 a.a.        Molecular weight: 9085.55 Da        Isoelectric Point: 4.6820

>AT45359 WP_000534857.1 NZ_CP006642:c43487-43248 [Escherichia coli PCN061]
MGSINLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPFKQTLLSDEDAELVEIVKERLRKPKPVRVTLDEL

Download         Length: 240 bp

>AT45359 NZ_CP006642:c43487-43248 [Escherichia coli PCN061]
ATGGGTAGCATTAACCTGCGTATTGACGATGAACTTAAAGCGCGTTCTTACGCCGCGCTTGAAAAAATGGGCGTAACTCC
TTCTGAAGCGCTTCGTCTCATGCTCGAGTATATCGCTGACAATGAACGCTTGCCGTTCAAACAGACACTCCTGAGTGATG
AAGATGCTGAACTTGTGGAGATAGTGAAAGAACGGCTTCGTAAGCCTAAGCCAGTACGTGTGACGCTGGATGAACTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4V7K
PDB 4V7J
PDB 4FXI
PDB 4FXE
PDB 2KC8
PDB 4FXH
PDB 2KC9

Antitoxin

Source ID Structure
AlphaFold DB A0A6C0NE60

References