Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4548953..4549529 Replicon chromosome
Accession NZ_CP006636
Organism Escherichia coli PCN061

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag PCN061_RS22810 Protein ID WP_001297643.1
Coordinates 4548953..4549240 (+) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag PCN061_RS22815 Protein ID WP_000063160.1
Coordinates 4549227..4549529 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PCN061_RS22785 4545190..4546470 - 1281 WP_001037390.1 DUF445 domain-containing protein -
PCN061_RS22790 4546511..4547743 - 1233 WP_001137030.1 multidrug efflux MFS transporter MdtM -
PCN061_RS27025 4548058..4548754 + 697 Protein_4287 IS1 family transposase -
PCN061_RS22810 4548953..4549240 + 288 WP_001297643.1 BrnT family toxin Toxin
PCN061_RS22815 4549227..4549529 + 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
PCN061_RS22820 4549563..4550519 - 957 WP_001297640.1 GTPase -
PCN061_RS22825 4550530..4550733 - 204 WP_000467859.1 YbdD/YjiX family protein -
PCN061_RS22830 4550783..4552933 - 2151 WP_001295524.1 pyruvate/proton symporter BtsT -
PCN061_RS28655 4553310..4553429 + 120 Protein_4293 Tar ligand binding domain-containing protein -
PCN061_RS22840 4553445..4554142 - 698 Protein_4294 IS1-like element IS1A family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - cheD 4548545..4557664 9119


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T45353 WP_001297643.1 NZ_CP006636:4548953-4549240 [Escherichia coli PCN061]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T45353 NZ_CP006636:4548953-4549240 [Escherichia coli PCN061]
ATGCCAATGGAGTTTGAATGGGATGAAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTATTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT45353 WP_000063160.1 NZ_CP006636:4549227-4549529 [Escherichia coli PCN061]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT45353 NZ_CP006636:4549227-4549529 [Escherichia coli PCN061]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCGATGTGCAGCATGAGGCCGAACTGAAGGCGCTGACGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCCGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References