Detailed information of TA system TA045348

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3137474..3138309
• Replicon: chromosome
• Accession: NZ_CP006636
• Organism: Escherichia coli PCN061

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A763DSF8
• Locus tag: PCN061_RS15835
• Protein ID: WP_047928871.1
• Coordinates: 3137474..3137851 (-)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: PCN061_RS15840
• Protein ID: WP_047928872.1
• Coordinates: 3137941..3138309 (-)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCN061_RS15805	3133399..3135240	+	1842	WP_000437375.1	RNA polymerase sigma factor RpoD	-
PCN061_RS15810	3135319..3135825	-	507	WP_000228937.1	G/U mismatch-specific DNA glycosylase	-
PCN061_RS15820	3136125..3136970	-	846	WP_047928869.1	DUF4942 domain-containing protein	-
PCN061_RS28520	3137055..3137252	-	198	WP_096093965.1	DUF957 domain-containing protein	-
PCN061_RS28525	3137328..3137477	-	150	Protein_2976	hypothetical protein	-
PCN061_RS15835	3137474..3137851	-	378	WP_047928871.1	TA system toxin CbtA family protein	Toxin
PCN061_RS15840	3137941..3138309	-	369	WP_047928872.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
PCN061_RS15845	3138359..3139003	-	645	WP_047928873.1	hypothetical protein	-
PCN061_RS15850	3139022..3139243	-	222	WP_000692327.1	DUF987 domain-containing protein	-
PCN061_RS15855	3139306..3139782	-	477	WP_001186771.1	RadC family protein	-
PCN061_RS15860	3139798..3140271	-	474	WP_001355870.1	antirestriction protein	-
PCN061_RS15865	3140340..3140609	-	270	WP_001183319.1	hypothetical protein	-
PCN061_RS15870	3140609..3141427	-	819	WP_047928874.1	DUF945 domain-containing protein	-
PCN061_RS15875	3141518..3141751	-	234	WP_047928875.1	DUF905 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13941.88 Da
• Isoelectric Point: 7.3222

>T45348 WP_047928871.1 NZ_CP006636:c3137851-3137474 [Escherichia coli PCN061]
MKTLPVLPGQAAGSRPSPVEIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
STCTHSQLINSIDILRARRATGLMTRDNYRTVNGITQGKHPEAKQ

Nucleotide

• Length: 378 bp

>T45348 NZ_CP006636:c3137851-3137474 [Escherichia coli PCN061]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCGGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCATTACGGCCTCACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCACCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCCCGCCGGGCGACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGGCATTACCCAGGGGAAGCATCCGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13757.47 Da
• Isoelectric Point: 5.0907

>AT45348 WP_047928872.1 NZ_CP006636:c3138309-3137941 [Escherichia coli PCN061]
VSDTLHETNYPDDNNDRPWWGLPCTVTPCFGARLVQEDNQLHYLADRAGIRGRFSDADAYHLDQALPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYMAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT45348 NZ_CP006636:c3138309-3137941 [Escherichia coli PCN061]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAAGACAACCAGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCACCTGGACCAAGCCCTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A763DSF8