Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2952688..2953342 Replicon chromosome
Accession NZ_CP006636
Organism Escherichia coli PCN061

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag PCN061_RS14825 Protein ID WP_000244781.1
Coordinates 2952688..2953095 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag PCN061_RS14830 Protein ID WP_000354046.1
Coordinates 2953076..2953342 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PCN061_RS14805 2948645..2950378 - 1734 WP_000813198.1 single-stranded-DNA-specific exonuclease RecJ -
PCN061_RS14810 2950384..2951094 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
PCN061_RS14815 2951119..2952015 - 897 WP_047928848.1 site-specific tyrosine recombinase XerD -
PCN061_RS14820 2952127..2952648 + 522 WP_001055874.1 flavodoxin FldB -
PCN061_RS14825 2952688..2953095 - 408 WP_000244781.1 protein YgfX Toxin
PCN061_RS14830 2953076..2953342 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
PCN061_RS14835 2953585..2954565 + 981 WP_000886083.1 tRNA-modifying protein YgfZ -
PCN061_RS14840 2954761..2955420 - 660 WP_000250274.1 hemolysin III family protein -
PCN061_RS14845 2955584..2955895 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
PCN061_RS14850 2955940..2957373 + 1434 WP_001336277.1 6-phospho-beta-glucosidase BglA -
PCN061_RS14855 2957430..2958173 - 744 WP_032298494.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T45346 WP_000244781.1 NZ_CP006636:c2953095-2952688 [Escherichia coli PCN061]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T45346 NZ_CP006636:c2953095-2952688 [Escherichia coli PCN061]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATAGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT45346 WP_000354046.1 NZ_CP006636:c2953342-2953076 [Escherichia coli PCN061]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT45346 NZ_CP006636:c2953342-2953076 [Escherichia coli PCN061]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTATGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTACTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References