Detailed information of TA system TA045333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 867890..868595
• Replicon: chromosome
• Accession: NZ_CP006636
• Organism: Escherichia coli PCN061

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: PCN061_RS04385
• Protein ID: WP_000539521.1
• Coordinates: 868209..868595 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: PCN061_RS04380
• Protein ID: WP_001280945.1
• Coordinates: 867890..868219 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
PCN061_RS04365	863047..863958	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
PCN061_RS04370	864136..866484	+	2349	WP_047928980.1	EAL domain-containing protein	-
PCN061_RS04375	866492..867820	+	1329	WP_000086877.1	GGDEF domain-containing protein	-
PCN061_RS04380	867890..868219	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
PCN061_RS04385	868209..868595	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
PCN061_RS04390	868821..870146	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
PCN061_RS04395	870359..870742	+	384	WP_000497137.1	biofilm formation regulator BssR	-
PCN061_RS04400	870853..871968	+	1116	Protein_828	aldose sugar dehydrogenase YliI	-
PCN061_RS04405	871965..872591	-	627	WP_001295292.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T45333 WP_000539521.1 NZ_CP006636:c868595-868209 [Escherichia coli PCN061]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T45333 NZ_CP006636:c868595-868209 [Escherichia coli PCN061]
ATGGGTGTGTATAAGGCAAGGCGATTCAGCCAAAGCACGAAAAAACTTGGTATTCACGATAAAGTTCTCATGGCTGCCGC
TGAAGAAGTGATGCAAGGCATATGGGAGGCTGATTTAGGTAGCGGTGTCATCAAAAAACGGTTACCCCTCCAACAAGGAA
AGAGTGGTGGAGCCAGAACAATTATCTTTTTTAAATCAGCAAATCATGTGTTTTTTTATGATGGTTGGTCCAAGAGTGGT
CTAAGCAGTAAAGGAACCAAAGAAATCGAAGATGATGAACTGGCAGCCTACAAAAAAATGGCAAATGCATTTCTGGCCTT
TAGCAATAAGCAAATAGAGGATTTGATCGAAACTGGCTTCTTGATTGAGGTGAAAAATGAGCGATAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT45333 WP_001280945.1 NZ_CP006636:c868219-867890 [Escherichia coli PCN061]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT45333 NZ_CP006636:c868219-867890 [Escherichia coli PCN061]
ATGAGCGATAAATTACAAATGATGCTGGAAGATGCAAAGGAACTGAATGCATTGGGCCTGATGTCTGATAGTGAGGTTGA
TGCCATTGCCGTCATGGTCAAAGCCCGTGAAGTCAGCGCCCGTATAGCCAAAGTCAAATCAATGACGGGTGAAGAGATAA
AAGCCATCCGCACCCGATACGGCATGTCACAATCCGTGCTGGCACATACAATGGGAATGTCAAAAGAAAGTGTCTCGAAA
TGGGAACGTAATGAAATAAAACCGAGCGGCCCGGCACTACGTATTTTGAATACATTGGCAATAAAAGGGCCGGAAGTTTT
TGCAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6