Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 506719..507337 Replicon chromosome
Accession NZ_CP006636
Organism Escherichia coli PCN061

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag PCN061_RS02605 Protein ID WP_001291435.1
Coordinates 506719..506937 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag PCN061_RS02610 Protein ID WP_000344800.1
Coordinates 506963..507337 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PCN061_RS02565 501834..502145 - 312 WP_000409911.1 MGMT family protein -
PCN061_RS02570 502524..502877 + 354 Protein_487 DUF1428 family protein -
PCN061_RS02575 502919..504085 - 1167 Protein_488 cyclic-guanylate-specific phosphodiesterase PdeB -
PCN061_RS02585 504141..504838 + 698 Protein_489 IS1-like element IS1A family transposase -
PCN061_RS02590 504854..505246 - 393 Protein_490 cyclic-guanylate-specific phosphodiesterase PdeB -
PCN061_RS02595 505410..505880 - 471 WP_000136192.1 YlaC family protein -
PCN061_RS02600 505996..506547 - 552 WP_000102564.1 maltose O-acetyltransferase -
PCN061_RS02605 506719..506937 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
PCN061_RS02610 506963..507337 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
PCN061_RS02615 507883..511032 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
PCN061_RS02620 511055..512248 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T45332 WP_001291435.1 NZ_CP006636:c506937-506719 [Escherichia coli PCN061]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T45332 NZ_CP006636:c506937-506719 [Escherichia coli PCN061]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT45332 WP_000344800.1 NZ_CP006636:c507337-506963 [Escherichia coli PCN061]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT45332 NZ_CP006636:c507337-506963 [Escherichia coli PCN061]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References